Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab22827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 28.7 kDa.
In Western Blot a 38kDa band was observed in Human Spleen and Bone Marrow lysates after 1µg/ml antibody staining. Please note that a band of similar size is reported in the literature (PMID 10383463). However, the calculated size is 28.7kDa according to NP_36374. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 18561248

Target

  • FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificityPlacenta.
  • Sequence similaritiesBelongs to the dickkopf family.
  • DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf related protein-1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • hDkk1 antibody
    • SK antibody
    see all

Anti-DKK1 antibody images

References for Anti-DKK1 antibody (ab22827)

This product has been referenced in:
  • Rachner TD  et al. Dickkopf-1 is regulated by the mevalonate pathway in breast cancer. Breast Cancer Res 16:R20 (2014). IHC-P ; Human . Read more (PubMed: 24528599) »
  • Hall CL  et al. Dickkopf-1 expression increases early in prostate cancer development and decreases during progression from primary tumor to metastasis. Prostate 68:1396-404 (2008). IHC-P ; Human . Read more (PubMed: 18561248) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"