Overview

  • Product nameAnti-DKK1 antibody
    See all DKK1 primary antibodies
  • Description
    Rabbit polyclonal to DKK1
  • Tested applicationsIHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) from the internal domain of DKK1 conjugated to KLH

  • Positive control
    • Human uterus, endometrium.

Properties

Applications

Our Abpromise guarantee covers the use of ab61034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes. Remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT.

Target

  • FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificityPlacenta.
  • Sequence similaritiesBelongs to the dickkopf family.
  • DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • hDkk1 antibody
    • SK antibody
    see all

Anti-DKK1 antibody images

  • ab61034 at 10µg/ml staining formalin fixed paraffin embedded Uterus (endometrium)
  • ab61034 staining DKK1 in mouse embryonic (E16.5) craniofacial tissue by IHC-Fr (paraformaldehyde-fixed frozen sections).  Paraformaldehyde fixed issue samples were permealized in 0.1% Triton X-100, blocked with 5% serum for 15 minutes at 25 °C. The sample was incubated with primary antibody dilution 1/100 in PBS at 4°C for 24 hours. An Alexa Fluor®  conjugated Goat polyclonal to rabbit IgG (dilution 1/200)  was used as secondary antibody.

    See Abreview

References for Anti-DKK1 antibody (ab61034)

This product has been referenced in:
  • Otto B  et al. Molecular changes in pre-metastatic lymph nodes of esophageal cancer patients. PLoS One 9:e102552 (2014). IHC-P ; Human . Read more (PubMed: 25048826) »
  • Ivanovska N & Dimitrova P Bone resorption and remodeling in murine collagenase-induced osteoarthritis after administration of glucosamine. Arthritis Res Ther 13:R44 (2011). IHC-P ; Mouse . Read more (PubMed: 21410959) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min at 100C
Sample Human Tissue sections (colon cancer)
Specification colon cancer
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

Submitted Dec 30 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, Dako, Citrate, Trypsin
Sample Mouse Tissue sections (E10.5, E15.5 embryos)
Specification E10.5, E15.5 embryos
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Antigen retrieval step Other
Sample Mouse Tissue sections (mice bone cell)
Specification mice bone cell
Permeabilization No
Fixative 10% neutral-buffered formalin
Username

Abcam user community

Verified customer

Submitted Jun 19 2013

Thank you for your reply. Some other options for positive controls include brain tissue (cerebellum), tonsil, ovary and testis. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results for your customer. The details provided will enable us to investigate this case and will provide us with vital ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Head of Mouse Embryos E16.5)
Specification Head of Mouse Embryos E16.5
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 (0.1%)
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gaskon Ibarretxe

Verified customer

Submitted Dec 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"