Anti-DKK1 antibody (ab88334)

Overview

• Product nameAnti-DKK1 antibodySee all DKK1 primary antibodies ...
• Description
  Mouse monoclonal to DKK1
• Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length Human protein (AAH01539), with a proprietary tag

• Positive controlWB: HeLa cells lysate. IHC: placenta tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Embryogenesis
  • Axis formation and symmetry

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Secreted

  • Cardiovascular
  • Blood
  • Other

  • Immunology
  • Immune Interventions
  • Transplantation

Applications

Target

• FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
• Tissue specificityPlacenta.
• Sequence similaritiesBelongs to the dickkopf family.
• DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
• Cellular localizationSecreted.

Target information above from: UniProt accession O94907 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 22943 Human
  • Omim: 605189 Human
  • SwissProt: O94907 Human
  • Unigene: 40499 Human

• Alternative names
  Dickkopf 1 antibodyDickkopf 1 homolog antibodyDickkopf 1 like antibody

  Dickkopf homolog 1 antibodyDickkopf like protein 1 antibodyDickkopf related protein 1 antibodyDickkopf-1 antibodyDickkopf-related protein 1 antibodyDKK 1 antibodyDkk-1 antibodyDkk1 antibodyDKK1_HUMAN antibodyhDkk 1 antibodyhDkk-1 antibodySK antibody

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Anti-DKK1 antibody images

• 

  Western blot - DKK1 antibody (ab88334)
  Anti-DKK1 antibody (ab88334) at 1 µg/ml + HeLa cell lysate at 25 µg
  
  Secondary
  Goat anti-Mouse IgG at 1/5000 dilution
  
  Predicted band size : 29 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DKK1 antibody (ab88334)
  Immunoperoxidase staining of DKK1 using ab88334 at 3 µg/ml on formalin-fixed, paraffin-embedded Human placenta tissue.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-DKK1 antibody (ab88334)
  ICC/IF image of ab88334 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88334, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.