DKK4 Human ELISA Kit (ab100503)
- Product nameDKK4 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range13.72 pg/ml - 10000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 109.6 97% - 121% Serum 95.59 85% - 105% Plasma 82.62 69% - 91%
- Species reactivityReacts with: Human
- Product overview
ab100503 Human DKK4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DKK4 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human DKK4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DKK4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent 5x concentrated buffer. For Standard/Sample (srum/ plasma samples/cell culture medium/urine) diluent. 1 x 15ml Detection Antibody Dkk-4: biotinylated anti-human Dkk-4 2 vials DKK 4 Standard Human(recombinant) 2 vials DKK4 Microplate (12 strips x 8 wells) coated with anti-human Dkk-4 1 unit Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- Dickkopf 4Dickkopf gene 4Dickkopf homolog 4
- Dickkopf related protein 4 Dickkopf4 DKK 4hDkk4MGC129562MGC129563MGC25705RGD1563172
- SwissProt: Q9UBT3 Human
Our Abpromise guarantee covers the use of ab100503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for DKK4 Human ELISA Kit (ab100503)
ab100503 has not yet been referenced specifically in any publications.