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Overview

  • Product nameDKK4 Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 109.6 97% - 121%
    Serum 95.59 85% - 105%
    Plasma 82.62 69% - 91%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100503 Human DKK4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DKK4 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human DKK4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DKK4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    200X HRP-Streptavidin Concentrate Item G 1 x 200µl
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent 5x concentrated buffer. For Standard/Sample (srum/ plasma samples/cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody Dkk-4: biotinylated anti-human Dkk-4 2 vials
    DKK 4 Standard Human(recombinant) 2 vials
    DKK4 Microplate (12 strips x 8 wells) coated with anti-human Dkk-4 1 unit
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • RelevanceDKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.
    • Alternative names
        Dickkopf 4Dickkopf gene 4Dickkopf homolog 4
        Dickkopf related protein 4 Dickkopf4 DKK 4hDkk4MGC129562MGC129563MGC25705RGD1563172
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100503 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      DKK4 Human ELISA Kit images

      • ELISA - DKK4 Human ELISA Kit (ab100503)
        ELISA - DKK4 Human ELISA Kit (ab100503)

      Protocols

      References for DKK4 Human ELISA Kit (ab100503)

      ab100503 has not yet been referenced specifically in any publications.

      Publishing research using ab100503 ? Please let us know so that we can cite the reference in this datasheet

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      Does your DKK4 Human ELISA Kit (ab100503) cross-react with mouse?

      Thank you for contacting us. Our testing data indicates that kit ab100503 shows less than 2% cross-reactivity with mouse. I hope this helps, please let me know if you need any additional information.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"