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Partial recombinant fragment with tag: HAPGDDLRPE ALPPDALISK IAIQGSLAVG QNWLLDEQTS TLTRLRYSYR VICSDNYYGD NCSRLCKKRN DHFGHYVCQP DGNLSCLPGW TGEYCQQPI, corresponding to amino acids 123-222 of Human DLL4
Our Abpromise guarantee covers the use of ab61031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 75 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. (methanol or paraformaldehyde fixed cells)|
|ICC/IF||Use at an assay dependent concentration.|
ab61031 staining DLL4 (red) in Human HUVEC endothelium cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 21°C. Samples were incubated with the primary antibody for 2 hours at 21°C. An undiluted Alexa Fluor®680-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody. Blue - nuclei.
Overlay histogram showing HEK293 cells stained with ab61031 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61031, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/250 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab61031 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"