Anti-DLL4 antibody (ab61031)

Overview

  • Product nameAnti-DLL4 antibodySee all DLL4 primary antibodies ...
  • Description
    Mouse monoclonal to DLL4
  • Tested applicationsWB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Partial recombinant fragment with tag: HAPGDDLRPE ALPPDALISK IAIQGSLAVG QNWLLDEQTS TLTRLRYSYR VICSDNYYGD NCSRLCKKRN DHFGHYVCQP DGNLSCLPGW TGEYCQQPI, corresponding to amino acids 123-222 of Human DLL4

Properties

Applications

Our Abpromise guarantee covers the use of ab61031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 75 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. (methanol or paraformaldehyde fixed cells)
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionPlays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • Tissue specificityExpressed in vascular endothelium.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 8 EGF-like domains.
  • DomainThe Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • Post-translational
    modifications
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delta 4 antibody
    • delta 4 precursor antibody
    • Delta ligand 4 antibody
    • delta ligand 4 precursor antibody
    • Delta like 4 antibody
    • Delta like 4 homolog antibody
    • Delta like 4 protein antibody
    • Delta like protein 4 antibody
    • Delta-like 4 (Drosophila) antibody
    • Delta-like protein 4 antibody
    • Delta4 antibody
    • DLL 4 antibody
    • DLL4 antibody
    • DLL4_HUMAN antibody
    • Drosophila Delta homolog 4 antibody
    • hdelta2 antibody
    • Homeobox protein DLL-4 antibody
    • MGC126344 antibody
    • Notch ligand delta 2 antibody
    • Notch ligand DLL4 antibody
    • notch ligand DLL4 precursor antibody
    • XDLL-4 antibody
    see all

Anti-DLL4 antibody images

  • ab61031 staining DLL4 (red) in Human HUVEC endothelium cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at 21°C. Samples were incubated with the primary antibody for 2 hours at 21°C. An undiluted Alexa Fluor®680-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody. Blue - nuclei.

    See Abreview



  • Predicted band size : 75 kDa
    Western blot against tagged recombinant protein immunogen using ab61031 DLL4 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.
  • Overlay histogram showing HEK293 cells stained with ab61031 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61031, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/250 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-DLL4 antibody (ab61031)

ab61031 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Human Cell (HUVEC Endothelium)
Specification HUVEC Endothelium
Permeabilization Yes - TritonX100 0,1% in PBS for 10 minutes
Fixative Formaldehyde
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Verified customer

Submitted Aug 15 2013

Thank you for contacting Abcam.

Please find attached the protocols that were used to test both ab7280 and ab61031 in an ELISA. For the HRP tagged secondary in the ab61031 protocol, you could use ab97080:

http://www.abcam.com/Goat-po...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"