Anti-DLL4 antibody (ab7280)

Overview

• Product nameAnti-DLL4 antibodySee all DLL4 primary antibodies ...
• Description
  Rabbit polyclonal to DLL4
• Tested applicationsIHC-P, WB, ICC/IF, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide: AWHAPGDDLRPEAL-C conjugated to KLH, corresponding to amino acids 121-134 of Human Delta4.

  Run BLAST with Run BLAST with
• Positive controlmouse pancreas

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Differentiation

  • Neuroscience
  • Neurology process
  • Notch Pathway

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionPlays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
• Tissue specificityExpressed in vascular endothelium.
• Sequence similaritiesContains 1 DSL domain.
  Contains 8 EGF-like domains.
• DomainThe Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
• Post-translational
  modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
• Cellular localizationMembrane.

Target information above from: UniProt accession Q9NR61 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 54567 Human
  • Entrez Gene: 54567 Human
  • Entrez Gene: 54485 Mouse
  • Omim: 605185 Human
  • SwissProt: Q9NR61 Human
  • SwissProt: Q9NR61 Human
  • SwissProt: Q9JI71 Mouse
  • Unigene: 511076 Human

  • Unigene: 143719 Mouse

  see all

• Alternative names
  Delta 4 antibodydelta 4 precursor antibodyDelta ligand 4 antibody

  delta ligand 4 precursor antibodyDelta like 4 antibodyDelta like 4 homolog antibodyDelta like 4 protein antibodyDelta like protein 4 antibodyDelta-like 4 (Drosophila) antibodyDelta-like protein 4 antibodyDelta4 antibodyDLL 4 antibodyDLL4 antibodyDLL4_HUMAN antibodyDrosophila Delta homolog 4 antibodyhdelta2 antibodyHomeobox protein DLL-4 antibodyMGC126344 antibodyNotch ligand delta 2 antibodyNotch ligand DLL4 antibodynotch ligand DLL4 precursor antibodyXDLL-4 antibody

  see all

Anti-DLL4 antibody images

• 

  Western blot - DLL4 antibody (ab7280)
  
  
  Predicted band size : 74 kDa
  
  
  Western blot using ab7280 at 1/500 on mouse Pancreas tissue. 20µg of cell lysate per lane. Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Exposure time: 3 minutes. Expected molecular weight: 74 kDa
• 

  Immunocytochemistry/ Immunofluorescence-DLL4 antibody(ab7280)
  ICC/IF image of ab7280 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-DLL4 antibody(ab7280)
  IHC image of ab7280 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7280, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.