Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab7280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/50.
WB 1/500 - 1/2500. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa).
ICC/IF Use at an assay dependent dilution.
ELISA 1/5000 - 1/25000.

Target

  • FunctionPlays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • Tissue specificityExpressed in vascular endothelium.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 8 EGF-like domains.
  • DomainThe Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • Post-translational
    modifications
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession Q9NR61 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Delta 4 antibody
    • delta 4 precursor antibody
    • Delta ligand 4 antibody
    • delta ligand 4 precursor antibody
    • Delta like 4 antibody
    • Delta like 4 homolog antibody
    • Delta like 4 protein antibody
    • Delta like protein 4 antibody
    • Delta-like 4 (Drosophila) antibody
    • Delta-like protein 4 antibody
    • Delta4 antibody
    • DLL 4 antibody
    • DLL4 antibody
    • DLL4_HUMAN antibody
    • Drosophila Delta homolog 4 antibody
    • hdelta2 antibody
    • Homeobox protein DLL-4 antibody
    • MGC126344 antibody
    • Notch ligand delta 2 antibody
    • Notch ligand DLL4 antibody
    • notch ligand DLL4 precursor antibody
    • XDLL-4 antibody
    see all

Anti-DLL4 antibody images



  • Predicted band size : 74 kDa


    Western blot using ab7280 at 1/500 on mouse Pancreas tissue. 20µg of cell lysate per lane. Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000) Exposure time: 3 minutes. Expected molecular weight: 74 kDa
  • ICC/IF image of ab7280 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7280, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab7280 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7280, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-DLL4 antibody (ab7280)

This product has been referenced in:
  • Harrison H  et al. Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling. Breast Cancer Res 15:R21 (2013). WB ; Human . Read more (PubMed: 23497505) »
  • Bazan-Peregrino M  et al. Combining virotherapy and angiotherapy for the treatment of breast cancer. Cancer Gene Ther 20:461-8 (2013). WB . Read more (PubMed: 23846253) »

See all 18 Publications for this product

Product Wall

Je peux vous confirmer que 0.2% Tween a été utilisé pour les immunoblots et que le lysat de pancréas de souris a été chauffé avant dépôt sur le gel de polyacrylamide.

Application Western blot
Sample Mouse Cell lysate - other (Lung Endothelial)
Loading amount 20 µg
Specification Lung Endothelial
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. VIctoria Thompson

Verified customer

Submitted May 09 2013


Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

Is the result as before: a strong band at the expected molecular weight of DLL4, and another smaller band at 72 kDa?

Can you tell me if the customer has tried any other DLL4 antibodies with these samples? Also, have they considered isolating...

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Thank you for contacting us.
Both of these antibodies are shipped at 4C on blue ice packs. They are both in stock, so if you order today by 8pm within the US, you will receive them by 10am tomorrow.
I hope this information is helpful to you. ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this...

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Thank you very much for your reply and your cooperation so far.

I have found your order for these two products, and would be happy to send you a replacement. Please let me know which products you have chosen from our catalog.

Thank you for contacting us again.

I am sorry to hear that the protocol tips did not improve the results. Please let me know when you have ordered the two antibodies. In addition, did you buy them directly from us or via our distributor?
<...

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Thank you very much for your cooperation so far and sending me the additional information.

I have one last tip i would like to share with you:

I would like to suggest to either decrease the H2O2 percentage down to 0.3% and to incubate...

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Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 25 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"