Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab82334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human DLL4 peptide (ab90637).

Target

  • FunctionPlays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4.
  • Tissue specificityExpressed in vascular endothelium.
  • Sequence similaritiesContains 1 DSL domain.
    Contains 8 EGF-like domains.
  • DomainThe Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor.
  • Post-translational
    modifications
    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Delta 4 antibody
    • delta 4 precursor antibody
    • Delta ligand 4 antibody
    • delta ligand 4 precursor antibody
    • Delta like 4 antibody
    • Delta like 4 homolog antibody
    • Delta like 4 protein antibody
    • Delta like protein 4 antibody
    • Delta-like 4 (Drosophila) antibody
    • Delta-like protein 4 antibody
    • Delta4 antibody
    • DLL 4 antibody
    • DLL4 antibody
    • DLL4_HUMAN antibody
    • Drosophila Delta homolog 4 antibody
    • hdelta2 antibody
    • Homeobox protein DLL-4 antibody
    • MGC126344 antibody
    • Notch ligand delta 2 antibody
    • Notch ligand DLL4 antibody
    • notch ligand DLL4 precursor antibody
    • XDLL-4 antibody
    see all

Anti-DLL4 antibody images

  • All lanes : Anti-DLL4 antibody (ab82334) at 1 µg/ml

    Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
    Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
    Lane 3 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References for Anti-DLL4 antibody (ab82334)

ab82334 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call today and for letting us know about the problem with ab7280. As we discussed, I am sending a free of charge vial of ab82334 on the order *** which should arrive on Monday. Please keep me updated about the results with th...

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