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Read our guarantee »Products:Signal Transduction >> Metabolism >> Amino Acids
Anti-DLST antibody
See all DLST products (4) ...
Mouse polyclonal to DLST
WB, ICC/IFmore details
Reacts with
Human
Full length Human DLST protein (NP_001924.2, 1 a.a. ~ 453 a.a).
Human liver lysate. DLST transfected 293T cell lysate. HeLa cells.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
Protein A purified
Polyclonal
IgG
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Metabolism >> Amino Acids
Our Abpromise guarantee covers the use of ab72790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 10 µg/ml. ab72790 requires purification with Protein A prior to ICC/IF.
WB: 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain.
Mitochondrion.
Target information above from: UniProt accessionP36957
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - DLST antibody (ab72790)

Anti-DLST antibody (ab72790) at 1/500 dilution + Human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 49 kDa
Observed band size : 49 kDa
Western blot - DLST antibody (ab72790)

All lanes : Anti-DLST antibody (ab72790) at 1/500 dilution
Lane 1 : DLST transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 49 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
ab72790 has not yet been referenced specifically in any publications.
Publishing research using ab72790? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-DLST antibody (ab72790) at 1/500 dilution + Human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 49 kDa
Observed band size : 49 kDa

All lanes : Anti-DLST antibody (ab72790) at 1/500 dilution
Lane 1 : DLST transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 49 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
0
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