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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-DLST antibody
See all DLST products (4) ...
Description
Mouse polyclonal to DLST
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length Human DLST protein (NP_001924.2, 1 a.a. ~ 453 a.a).
Positive control
Human liver lysate. DLST transfected 293T cell lysate. HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
ICC/IF
Application notes
ICC/IF: Use at a concentration of 10 µg/ml. ab72790 requires purification with Protein A prior to ICC/IF.
WB: 1/500 - 1/1000. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Pathway
Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.
Sequence similarities
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain.
Cellular localization
Mitochondrion.
Target information above from: UniProt accessionP36957
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex antibody
- mitochondrial antibody
- 2-oxoglutarate dehydrogenase complex component E2 antibody
see all
Database links
- Entrez Gene: 1743 Human
- Omim: 126063 Human
- SwissProt: P36957 Human
- Unigene: 525459 Human
Anti-DLST antibody images:
Western blot - DLST antibody (ab72790)
Anti-DLST antibody (ab72790) at 1/500 dilution + Human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 49 kDa
Observed band size : 49 kDa
Western blot - DLST antibody (ab72790)
All lanes : Anti-DLST antibody (ab72790) at 1/500 dilution
Lane 1 : DLST transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 49 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
References for Anti-DLST antibody (ab72790)
ab72790 has not yet been referenced specifically in any publications.
Publishing research using ab72790? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"