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Product name
Anti-DMAP1 antibody
See all DMAP1 products (10) ...
Description
Goat polyclonal to DMAP1
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Zebrafish
Immunogen
Synthetic peptide (Human) - which represented a portion of human DNA methyltransferase 1-associated protein 1 encoded within exon 8.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Involved in transcription repression. Its interaction with HDAC2 may provide a platform for how histones may become deacetylated in heterochromatin following replication. Can repress transcription independently of histone deacetylase activity.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200.
WB: 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
Target
Relevance
DMAP1 is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
Cellular localization
Nuclear
Alternative names
- DKFZp686L09142 antibody
- DMAP 1 antibody
- DNA methyltransferase 1-associated protein 1 antibody
see all
Database links
- Entrez Gene: 55929 Human
- Entrez Gene: 66233 Mouse
- Entrez Gene: 393225 Zebrafish
- Omim: 605077 Human
- SwissProt: Q9NPF5 Human
- SwissProt: Q9JI44 Mouse
- SwissProt: A2ADW4 Mouse
- Unigene: 8008 Human
Anti-DMAP1 antibody images:
Western blot - DMAP1 antibody (ab3737)
Predicted band size : 65 kDa
Sample: Hela cell lysate (50
Antibody: Affinity purified Goat anti-DMAP 1 (ab3737) used at the concentrations indicated.
Detection: ECL exposed for 2 minutes.
Immunocytochemistry/ Immunofluorescence - DMAP1 antibody (ab3737)
ab3737 (1/200) staining DMAP1 in assynchronous HeLa cells. Cells were fixed in paraformaldehyde and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
References for Anti-DMAP1 antibody (ab3737)
ab3737 has not yet been referenced specifically in any publications.
Publishing research using ab3737? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"