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Anti-DMP1 antibody (ab76632) at 1 µg/ml + Recombinant tagged human DMP1 fragment at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size : 56 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 38kDa.
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