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- Secondary antibodies
- Isotype controls
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Product name
Anti-DMT1 antibody
See all DMT1 products (5) ...
Description
Mouse monoclonal to DMT1
Tested applications
Flow Cyt, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: MVLGPEQKMSDDSVSGDHGESASLGNINPYSNPSLSQSPG DSEEYFATYFNEKISIPEEEYSCF, corresponding to amino acids 1-66 of Human DMT1
MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSP GDSEEYFATYFNEKISIPEEEYSCF
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Signal Transduction >> Metabolism >> Vitamins / Minerals
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 1µg for 106 cells.
IHC-P: Use a concentration of 3 µg/ml.
WB: Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Target
Function
Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution.
Tissue specificity
Ubiquitously expressed. Isoform 1 is highly expressed in brain. Isoform 2 is highly expressed in spleen, thymus and pancreas. Isoform 3 and isoform 4 are abundantly expressed in duodenum and kidney.
Involvement in disease
Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA) [MIM:206100]. The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels.
Sequence similarities
Belongs to the NRAMP family.
Post-translational
modifications
Ubiquitinated by WWP2.
Cellular localization
Endosome membrane.
Target information above from: UniProt accessionP49281
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCT 1 antibody
- DCT1 antibody
- Divalent cation transporter 1 antibody
see all
Database links
- Entrez Gene: 4891 Human
- Omim: 600523 Human
- SwissProt: P49281 Human
- Unigene: 505545 Human
Anti-DMT1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DMT1 antibody (ab55735)
DMT1 antibody (ab55735) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.
Western blot - DMT1 antibody (ab55735)
Western blot against tagged recombinant protein immunogen using ab55735 DMT1 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa
Flow Cytometry-DMT1 antibody(ab55735)
Overlay histogram showing SH-SY5Y cells stained with ab55735 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55735, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-DMT1 antibody (ab55735)
ab55735 has not yet been referenced specifically in any publications.
Publishing research using ab55735? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"