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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab75596 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - DNA Gyrase B antibody (ab75596)
All lanes : Anti-DNA Gyrase B antibody (ab75596) at 1/1000 dilution
Lane 1 : E. coli cell lysate (Gyrase A, whole protein) at 0.1 µg
Lane 2 : E. coli cell lysate (Gyrase A, whole protein) at 0.5 µg
Lane 3 : E. coli cell lysate (Gyrase A, whole protein) at 1 µg
Lane 4 : E. coli cell lysate (Gyrase B, whole protein) at 0.1 µg
Lane 5 : E. coli cell lysate (Gyrase B, whole protein) at 0.5 µg
Lane 6 : E. coli cell lysate (Gyrase B, whole protein) at 1 µg
Lane 7 : E. coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
Lane 8 : E. coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
Secondary
goat anti rabbit Igs at 1/5000 dilution
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
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