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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
Overview
Product name
Anti-DNA Gyrase B antibody
See all DNA Gyrase B products (3) ...
Description
Rabbit polyclonal to DNA Gyrase B
Tested applications
Cross reactivity
Reacts with
Escherichia coli , Staphylococcus aureus
Immunogen
Purified E. coli Gyr B protein (over expressed from E. coli)
Positive control
E. coli cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
This antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Topoisomerases
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Western blot - DNA Gyrase B antibody (ab75596)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000.
Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed gyrase in cell extracts.
The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
DNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Alternative names
- acrB antibody
- DNA gyrase subunit B antibody
- himB antibody
see all
Database links
- SwissProt: P0AES6 Escherichia coli
- SwissProt: P0A0K8 Staphylococcus aureus
Anti-DNA Gyrase B antibody images:
Western blot - DNA Gyrase B antibody (ab75596)
All lanes : Anti-DNA Gyrase B antibody (ab75596) at 1/1000 dilution
Lane 1 : E. coli cell lysate (Gyrase A, whole protein) at 0.1 µg
Lane 2 : E. coli cell lysate (Gyrase A, whole protein) at 0.5 µg
Lane 3 : E. coli cell lysate (Gyrase A, whole protein) at 1 µg
Lane 4 : E. coli cell lysate (Gyrase B, whole protein) at 0.1 µg
Lane 5 : E. coli cell lysate (Gyrase B, whole protein) at 0.5 µg
Lane 6 : E. coli cell lysate (Gyrase B, whole protein) at 1 µg
Lane 7 : E. coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
Lane 8 : E. coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
Secondary
goat anti rabbit Igs at 1/5000 dilution
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
References for Anti-DNA Gyrase B antibody (ab75596)
ab75596 has not yet been referenced specifically in any publications.
Publishing research using ab75596? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"