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Western blot - DNA Ligase IV antibody (ab26039)
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Immunohistochemistry (Paraffin-embedded sections) - DNA Ligase IV antibody (ab26039)
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Product Name
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DNA Ligase IV antibody
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See all DNA Ligase IV antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DNA Ligase IV
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Immunogen
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A region within a synthetic peptide: IADIEHIEKD MKHQSFYIET KLDGERMQMH KDGDVYKYFS RNGYNYTDQF, corresponding to internal sequence amino acids 253-302 of DNA Ligase IV
BLAST 'IADIEHIEKD MKHQSFYIET KLDGERMQMH KDGDVYKYFS RNGYNYTDQF ' with  or 
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Reacts with
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Hu
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Tested applications
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IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
IP: Use at an assay dependent dilution (PMID 18451142).
WB: Use at a concentration of 1.25 µg/ml. Predicted molecular weight: 109 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Ligases
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Relevance
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DNA Ligase IV exists as a complex with XRCC4 and plays an important role in V(D)J recombination and non-homologous end-joining (NHEJ) of DNA double strand breaks. DNA Ligase IV joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. It interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.00 - 2.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DNA Ligase IV antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DNA Ligase IV antibody images, references, products related to ab26039 and other tools.
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DNA Ligase IV antibody images:
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 Western blot - DNA Ligase IV antibody (ab26039)
DNA Ligase IV antibody (ab26039) at 1.25 µg/ml + HepG2 cell lysate
Predicted band size : 109 kDa
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Immunohistochemistry (Paraffin-embedded sections) - DNA Ligase IV antibody (ab26039)
ab26039 at a concentration of 6.0 µg/ml staining human DNA Ligase IV by Immunohistochemistry in paraffin embedded pancreas tissue. Cells with positive label (as indicated by the arrows) are epithelial cells of the pancreatic acinus. Magnification 400X.
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References for DNA Ligase IV antibody (ab26039)
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This product has been used in:
Chen X et al. Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair. Cancer Res 68:3169-77 (2008). IP; Human. PubMed: 18451142
If you publish research using ab26039 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to DNA Ligase IV
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DNA Ligase IV antibody - more information
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Customer reviews (feedback) regarding DNA Ligase IV antibody |
Customer FAQs regarding DNA Ligase IV antibody |
Protocols for DNA Ligase IV antibody |
Price and availability of products related to DNA Ligase IV antibody |
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DNA Ligase IV antibody - related products:
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DNA Ligase IV antibody - other tools:
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Contact Abcam with a Technical Enquiry about DNA Ligase IV antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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