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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - DNA Ligase IV antibody (ab65130)
All lanes : Anti-DNA Ligase IV antibody (ab65130) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells, with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 104 kDa
Observed band size : 104 kDa
Additional bands at : 28 kDa,48-55 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
Immunocytochemistry/ Immunofluorescence-DNA Ligase IV antibody(ab65130)
ICC/IF image of ab65130 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65130, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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