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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Ligases
Overview
Product name
Anti-DNA Ligase IV antibody
See all DNA Ligase IV products (3) ...
Description
Rabbit polyclonal to DNA Ligase IV
Tested applications
ELISA, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
A synthesized peptide derived from an internal region of human DNA Ligase IV
Positive control
Extracts from Jurkat cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Ligases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Tissue specificity
Testis, thymus, prostate and heart.
Involvement in disease
Defects in LIG4 are the cause of LIG4 syndrome (LIG4S) [MIM:606593]. This disease is characterized by immunodeficiency and developmental and growth delay. Patients display unusual facial features, microcephaly, growth and/or developmental delay, pancytopenia, and various skin abnormalities.
Defects in LIG4 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint formation and the completion of V(D)J recombination. A subset of cells from such patients show increased radiosensitivity.
Sequence similarities
Belongs to the ATP-dependent DNA ligase family.
Contains 2 BRCT domains.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP49917
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA ligase IV ATP dependent antibody
- DNA joinase antibody
- DNA ligase 4 antibody
see all
Database links
- Entrez Gene: 3981 Human
- Omim: 601837 Human
- SwissProt: P49917 Human
- Unigene: 166091 Human
Anti-DNA Ligase IV antibody images:
Western blot - DNA Ligase IV antibody (ab65130)
All lanes : Anti-DNA Ligase IV antibody (ab65130) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from Jurkat cells, with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 104 kDa
Observed band size : 104 kDa
Additional bands at : 28 kDa,48-55 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
Immunocytochemistry/ Immunofluorescence-DNA Ligase IV antibody(ab65130)
ICC/IF image of ab65130 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-DNA Ligase IV antibody (ab65130)
ab65130 has not yet been referenced specifically in any publications.
Publishing research using ab65130? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"