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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Ligases
Anti-DNA Ligase IV antibody (ab80514)
Product partner
Related products
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Proteins and Peptides
ab90599
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab80514? Please let us know so that we can cite the reference in this datasheet
ab80514 has been referenced in 1 publications.
- Gao Y et al. DNA ligase III is critical for mtDNA integrity but not Xrcc1-mediated nuclear DNA repair. Nature 471:240-4 (2011). WB; Mouse. PubMed: 21390131
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - DNA Ligase IV antibody (ab80514)
All lanes : Anti-DNA Ligase IV antibody (ab80514) at 1 µg/ml
Lane 1 :
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 103 kDa
Observed band size : 103 kDa
Additional bands at : 18 kDa,78 kDa,87 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Immunocytochemistry/ Immunofluorescence - DNA Ligase IV antibody (ab80514)
ICC/IF image of ab80514 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80514, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DNA Ligase IV antibody (ab80514)
IHC image of ab80514 staining in Human Normal Cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80514, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-DNA Ligase IV antibody for Immunocytochemistry/ Immunofluorescence in Human (80514)
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