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Human DNA-dependent Protein Kinase purified from HeLa cells.
Our Abpromise guarantee covers the use of ab1832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0,for 10-20 min followed by cooling at RT for 20 min.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. (protein G) Use at a concentration of 2µg/mg of protein lysate (native and denatured).|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 460 kDa (predicted molecular weight: 469 kDa). This antibody reacts with intact DNA-PKcs as well as its degradation products.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20709753|
|Flow Cyt||Use at an assay dependent concentration. Ab81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"