Anti-DNA PKcs antibody [18-2] (ab1832)

Overview

• Product nameAnti-DNA PKcs antibody [18-2]See all DNA PKcs primary antibodies ...
• Description
  Mouse monoclonal [18-2] to DNA PKcs
• SpecificityThis antibody is specific to 460 kDa protein known as DNA-dependent Protein Kinase (DNA-PKcs).
• Tested applicationsFunctional Studies, IHC-P, IHC-Fr, IP, WB, ICC/IF, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Human DNA-dependent Protein Kinase purified from HeLa cells.

• EpitopeAmino acids 1-2713.
• Positive controlLS174T cells. Tonsil.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number18-2
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Non Homol. End Joining

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • DNA Damage Recognition

Applications

Target

• FunctionSerine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
• Sequence similaritiesBelongs to the PI3/PI4-kinase family.
  Contains 1 FAT domain.
  Contains 1 FATC domain.
  Contains 2 HEAT repeats.
  Contains 1 PI3K/PI4K domain.
  Contains 3 TPR repeats.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
  S-nitrosylated by GAPDH.
• Cellular localizationNucleus.

Target information above from: UniProt accession P78527 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5591 Human
  • Entrez Gene: 19090 Mouse
  • Entrez Gene: 360748 Rat
  • Omim: 600899 Human
  • SwissProt: P78527 Human
  • SwissProt: P97313 Mouse
  • Unigene: 491682 Human
  • Unigene: 71 Mouse

• Alternative names
  DNA dependent protein kinase catalytic subunit antibodyDNA PK catalytic subunit antibodyDNA-dependent protein kinase catalytic subunit antibody

  DNA-PK catalytic subunit antibodyDNA-PKcs antibodyDNAPK antibodyDNAPK catalytic subunit antibodyDNPK 1 antibodyDNPK1 antibodyDNPK1 antibodyHyper radiosensitivity of murine scid mutation, complementing 1 antibodyHYRC 1 antibodyHYRC antibodyHYRC1 antibodyHYRC1 antibodyp350 antibodyp460 antibodyPKRDC antibodyPRKDC antibodyPRKDC_HUMAN antibodyProtein Kinase DNA Activated Catalytic Polypeptide antibodyProtein Kinase DNA Activated Catalytic Polypeptide antibodyXRCC 7 antibodyXRCC7 antibodyXRCC7 antibody

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Anti-DNA PKcs antibody [18-2] images

• 

  Immunocytochemistry/ Immunofluorescence - DNA PKcs antibody [18-2] (ab1832)Image from Qi Y et al, J Biol Chem. 2010 Oct 22;285(43):33010-7. Epub 2010 Aug 6, Fig 5. DOI 10.1074/jbc.M110.137406
  ab1832 staining DNA PKcs in HCT 116 cells by Immunocytochemistry/ Immunofluorescence.
  
  The cells were washed in PBS, fixed in 4% paraformaldehyde (in PBS) for 15 minutes, permeabilized in 0.3% Triton X-100 at room temperature for 10 minutes, followed by overnight incubation of ab1832 at a 1/500 dilution.
• 

  Flow Cytometry - Anti-DNA PKcs antibody [18-2] (ab1832)This image is courtesy of an Anonymous Abreview.
  Overlay histogram showing Jurkat cells stained with ab1832 (blue line). The cells were prepared as follows: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight. Buffer was was 0.1% sodium azide with FBS in PBS. Cells fixed with paraformaldehyde and then permeabilized with Triton X-100 and NP40. Cell population was gated by isolating cell population from plot of SSC-A / FSA-A. The cells were then incubated the antibody at 1/100 for 24 hours. The secondary antibody used was FITC-conjugated Goat anti-Mouse polyclonal at 1/100 dilution. Isotype control antibody (red line) was used under the same conditions

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DNA PKcs antibody [18-2] (ab1832)
  Anti-DNA PKcs antibody ab1832 used to stain Human tonsil tissue. The antibody requires antigen retrieval with citrate buffer and we recommend a 1:50-1:100 dilution with a 30 minute incubation time of the antibody at room temperature.