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Anti-DNA PKcs antibody [18-2] (ab44815)

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Overview

  • Product nameAnti-DNA PKcs antibody [18-2]See all DNA PKcs primary antibodies ...
  • Description
    Mouse monoclonal [18-2] to DNA PKcs
  • Tested applicationsInhibition Assay, ICC/IF, IP, WB, IHC-P more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length native protein (purified from HeLa cells), corresponding to amino acids 1-2713 of Human DNA PKcs

  • Epitopeamino acids 1-2713
  • Positive controlLS174T cells. Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab44815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Inhibition Assay
ICC/IF
IP
WB
IHC-P
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: 1/400.
    IP: 1/400.
    Inhib (Inhibits (50%) DNA-dependent Phosphorylation): Use at an assay dependent dilution.
    WB: 1/200. Predicted molecular weight: 469 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionSerine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
      Contains 3 TPR repeats.
    • Post-translational
      modifications      Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
      S-nitrosylated by GAPDH.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession P78527 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          DNA dependent protein kinase catalytic subunit antibodyDNA PK catalytic subunit antibodyDNA-dependent protein kinase catalytic subunit antibody
          DNA-PK catalytic subunit antibodyDNA-PKcs antibodyDNAPK antibodyDNAPK catalytic subunit antibodyDNPK 1 antibodyDNPK1 antibodyHyper radiosensitivity of murine scid mutation, complementing 1 antibodyHYRC 1 antibodyHYRC antibodyHYRC1 antibodyp350 antibodyp460 antibodyPKRDC antibodyPRKDC antibodyPRKDC_HUMAN antibodyProtein Kinase DNA Activated Catalytic Polypeptide antibodyXRCC 7 antibodyXRCC7 antibody
        see all

      References for Anti-DNA PKcs antibody [18-2] (ab44815)

      ab44815 has not yet been referenced specifically in any publications.

      Publishing research using ab44815 ? Please let us know so that we can cite the reference in this datasheet

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      Hi – we’re looking at possible cleavage of human DNA-PK, we recognize a smaller fragment with an antibody to the C-terminus, would be helpful to have an antibody to recognize the other cleavage product which is likely the N-terminus. Can you recommend an ...

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      Thank you for contacting us regarding this. I've spoken with the lab regarding our Anti-DNA PKcs antibodies and have found that  ab1832 would most likely fulfill your needs as it's epitope site is between  aa1-2713. I hope this information is helpful to y...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"