Overview

  • Product nameAnti-DNA PKcs antibody [Y393]
    See all DNA PKcs primary antibodies
  • Description
    Rabbit monoclonal [Y393] to DNA PKcs
  • SpecificityThis antibody is specific for DNA PKcs. It may also detect the splice isoform 2.
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Armenian Hamster
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of Human DNA PKcs.

  • Positive control
    • WB: K562 cell lysate. ICC/IF: Hela cells. IHC-P: Human breast carcinoma tissue slides.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab32566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: 1/250 - 1/500.
    IHC-P: 1/50.
    WB: 1/1000. Detects a band of approximately 460 kDa (predicted molecular weight: 469 kDa).

    Is unsuitable for FACS or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
      Contains 3 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
      S-nitrosylated by GAPDH.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA dependent protein kinase catalytic subunit antibody
      • DNA PK catalytic subunit antibody
      • DNA-dependent protein kinase catalytic subunit antibody
      • DNA-PK catalytic subunit antibody
      • DNA-PKcs antibody
      • DNAPK antibody
      • DNAPK catalytic subunit antibody
      • DNPK 1 antibody
      • DNPK1 antibody
      • Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
      • HYRC 1 antibody
      • HYRC antibody
      • HYRC1 antibody
      • p350 antibody
      • p460 antibody
      • PKRDC antibody
      • PRKDC antibody
      • PRKDC_HUMAN antibody
      • Protein Kinase DNA Activated Catalytic Polypeptide antibody
      • XRCC 7 antibody
      • XRCC7 antibody
      see all

    Anti-DNA PKcs antibody [Y393] images

    • Anti-DNA PKcs antibody [Y393] (ab32566) at 1/1000 dilution + K562 cell lysate.

      Predicted band size : 469 kDa
      Observed band size : 460 kDa (why is the actual band size different from the predicted?)
    • Ab32566, at a 1/50 dilution, staining DNA PKcs in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    • Ab32566, at a 1/250 dilution, staining DNA PKcs in Hela cells by Immunocytochemistry/Immunofluorescence.

    References for Anti-DNA PKcs antibody [Y393] (ab32566)

    This product has been referenced in:
    • Yüce Ö & West SC Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response. Mol Cell Biol 33:406-17 (2013). WB . Read more (PubMed: 23149945) »
    • Wang Y  et al. MicroRNA-138 Modulates DNA Damage Response by Repressing Histone H2AX Expression. Mol Cancer Res 9:1100-11 (2011). WB ; Human . Read more (PubMed: 21693595) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 20 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Submitted Dec 30 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (breast cancer cell)
    Specification breast cancer cell
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% triton X100
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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    Submitted May 09 2008

    Thank you for contacting us and your interest in our products.

    Epitomics did offer isotype controls specific for different lots of antibody purchased. This was providing the antibody at the same concentration as the antibody to be tested. We d...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HEK293)
    Loading amount 200000 cells
    Specification HEK293
    Gel Running Conditions Reduced Denaturing (5% gel)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Submitted Feb 02 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549 tumour cell line)
    Loading amount 50 µg
    Specification A549 tumour cell line
    Treatment 100IU bleomycin for 2h
    Gel Running Conditions Reduced Denaturing (3-8% tris acetate gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Submitted Nov 27 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (mouse muscle)
    Loading amount 100 µg
    Specification mouse muscle
    Gel Running Conditions Reduced Denaturing (6% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Sep 14 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Armenian Hamster Tissue lysate - whole (Liver tissue)
    Loading amount 50 µg
    Specification Liver tissue
    Gel Running Conditions Reduced Denaturing (6% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Sep 14 2009

    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (COS-7 cell line)
    Loading amount 20 µg
    Specification COS-7 cell line
    Gel Running Conditions Reduced Denaturing (6% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Sep 11 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Cell lysate - whole cell (C6 Cell line)
    Loading amount 25 µg
    Specification C6 Cell line
    Gel Running Conditions Reduced Denaturing (6% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Jun 11 2008

    Application Immunoprecipitation
    Sample Human Cell lysate - nuclear (Breast cancer cells)
    Total protein in input 200 µg
    Specification Breast cancer cells
    Immuno-precipitation step Protein A/G
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    Submitted May 21 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"