Overview

  • Product nameAnti-DNA PKcs antibody
    See all DNA PKcs primary antibodies
  • Description
    Rabbit polyclonal to DNA PKcs
  • SpecificityStrong reactivity with human DNA PKcs.
  • Tested applicationsIHC-P, ICC/IF, WB, IP, Gel supershift assaysmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
  • Immunogen

    Synthetic peptide (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
WB
IP
Gel supershift assays
  • Application notesGSA: Use at an assay dependent dilution.
    ICC/IF: 1/200.
    IHC-P: 1/100.
    IP: Use at an assay dependent dilution.
    WB: 1/2000. Predicted molecular weight: 469 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 2 HEAT repeats.
      Contains 1 PI3K/PI4K domain.
      Contains 3 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
      S-nitrosylated by GAPDH.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DNA dependent protein kinase catalytic subunit antibody
      • DNA PK catalytic subunit antibody
      • DNA-dependent protein kinase catalytic subunit antibody
      • DNA-PK catalytic subunit antibody
      • DNA-PKcs antibody
      • DNAPK antibody
      • DNAPK catalytic subunit antibody
      • DNPK 1 antibody
      • DNPK1 antibody
      • DNPK1 antibody
      • Hyper radiosensitivity of murine scid mutation, complementing 1 antibody
      • HYRC 1 antibody
      • HYRC antibody
      • HYRC1 antibody
      • HYRC1 antibody
      • p350 antibody
      • p460 antibody
      • PKRDC antibody
      • PRKDC antibody
      • PRKDC_HUMAN antibody
      • Protein Kinase DNA Activated Catalytic Polypeptide antibody
      • Protein Kinase DNA Activated Catalytic Polypeptide antibody
      • XRCC 7 antibody
      • XRCC7 antibody
      • XRCC7 antibody
      see all

    Anti-DNA PKcs antibody images

    • ICC/IF image of ab230 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab230, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-DNA PKcs antibody (ab230)

    This product has been referenced in:
    • Moeller BJ  et al. Discovery of DNA repair inhibitors by combinatorial library profiling. Cancer Res 71:1816-24 (2011). WB, ICC/IF . Read more (PubMed: 21343400) »
    • Hoshino A  et al. Regulation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase. J Biol Chem 282:17706-11 (2007). WB ; Mouse . Read more (PubMed: 17446165) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Blocking step 0,25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
    Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
    Specification mouse embryonic spinal cord E11
    Permeabilization Yes - 0,25%TritonX100
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 07 2014

    As far as we are aware, ab230 will still bind DNA-PKcs in the presence of the Ku subunits. However, the customer should note that DNA-PKcs is not normally tightly associated with Ku and only interacts with Ku in a significant manner when there are D...

    Read More

    This antibody was raised against the 2790-3065aa fragment of the DNA-PKcs protein.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"