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Product name
Anti-DNA PKcs antibody
See all DNA PKcs products (22) ...
Description
Rabbit polyclonal to DNA PKcs
Specificity
Strong reactivity with human DNA PKcs.
Tested applications
IHC-P, ICC/IF, WB, IP, Gel supershift assaysmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Human
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.1% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
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Immunocytochemistry/ Immunofluorescence - DNA PKcs antibody (ab230)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
- 1 Image
ICC/IF
IP
Gel supershift assays
Application notes
GSA: Use at an assay dependent dilution.
ICC/IF: 1/200.
IHC-P: 1/100.
IP: Use at an assay dependent dilution.
WB: 1/2000. Predicted molecular weight: 469 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 2 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Contains 3 TPR repeats.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
S-nitrosylated by GAPDH.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP78527
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA dependent protein kinase catalytic subunit antibody
- DNA PK catalytic subunit antibody
- DNA-dependent protein kinase catalytic subunit antibody
see all
Database links
- Entrez Gene: 5591 Human
- Entrez Gene: 19090 Mouse
- Entrez Gene: 360748 Rat
- Omim: 600899 Human
- SwissProt: P78527 Human
- SwissProt: P97313 Mouse
- Unigene: 415749 Human
- Unigene: 491682 Human
see all
Anti-DNA PKcs antibody images:
Immunocytochemistry/ Immunofluorescence - DNA PKcs antibody (ab230)
ICC/IF image of ab230 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab230, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-DNA PKcs antibody (ab230)
This product has been referenced in:
- Moeller BJ et al. Discovery of DNA repair inhibitors by combinatorial library profiling. Cancer Res 71:1816-24 (2011). WB, ICC/IF.Read more (PubMed: 21343400) »
- Hoshino A et al. Regulation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase. J Biol Chem 282:17706-11 (2007). WB; Mouse.Read more (PubMed: 17446165) »
See all 6 publications for this product
Publishing research using ab230? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"