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Synthetic peptide conjugated to KLH derived from within residues 3650 - 3750 of Human DNA PKcs.
(Peptide available as ab41959.)
Our Abpromise guarantee covers the use of ab39978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 460 kDa (predicted molecular weight: 469 kDa).Can be blocked with Human DNA PKcs peptide (ab41959).|
|ICC/IF||Use a concentration of 1 µg/ml.|
DNA PKcs was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to DNA PKcs and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab39978.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to anti-Rabbit IgG light chain HRP (ab99697).
Band: 460kDa: DNA PKcs.
ab39978 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"