Anti-DNA PKcs antibody (ab53701)

Overview

• Product nameAnti-DNA PKcs antibodySee all DNA PKcs primary antibodies ...
• Description
  Rabbit polyclonal to DNA PKcs
• Specificityab53701 detects endogenous levels of total DNA PKcs protein.
• Tested applicationsELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide derived from human DNA PKcs.

• Positive controlHuman breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Non Homol. End Joining

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • DNA Damage Recognition

Applications

Target

• FunctionSerine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
• Sequence similaritiesBelongs to the PI3/PI4-kinase family.
  Contains 1 FAT domain.
  Contains 1 FATC domain.
  Contains 2 HEAT repeats.
  Contains 1 PI3K/PI4K domain.
  Contains 3 TPR repeats.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
  S-nitrosylated by GAPDH.
• Cellular localizationNucleus.

Target information above from: UniProt accession P78527 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5591 Human
  • Entrez Gene: 19090 Mouse
  • Omim: 600899 Human
  • SwissProt: P78527 Human
  • SwissProt: P97313 Mouse
  • Unigene: 491682 Human
  • Unigene: 71 Mouse

• Alternative names
  DNA dependent protein kinase catalytic subunit antibodyDNA PK catalytic subunit antibodyDNA-dependent protein kinase catalytic subunit antibody

  DNA-PK catalytic subunit antibodyDNA-PKcs antibodyDNAPK antibodyDNAPK catalytic subunit antibodyDNPK 1 antibodyDNPK1 antibodyHyper radiosensitivity of murine scid mutation, complementing 1 antibodyHYRC 1 antibodyHYRC antibodyHYRC1 antibodyp350 antibodyp460 antibodyPKRDC antibodyPRKDC antibodyPRKDC_HUMAN antibodyProtein Kinase DNA Activated Catalytic Polypeptide antibodyXRCC 7 antibodyXRCC7 antibody

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Anti-DNA PKcs antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - DNA PKcs antibody (ab53701)
  ab53701 at 1/50 dilution staining DNA PKcs in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DNA PKcs antibody (ab53701)This image is courtesy of an anonymous Abreview.
  ab53701 at 1/50 dilution staining DNA PKs in human FFPE Hela cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 5% serum for 15 minutes at room temperature and then incubated with ab53701 for 1 hour. A donkey polycolnal conjugated with HRP was used as the secondary antibody.

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