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Overview

  • Product nameAnti-DNA PKcs antibodySee all DNA PKcs primary antibodies ...
  • Description
    Rabbit polyclonal to DNA PKcs
  • Tested applicationsICC/IF, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non phosphopeptide (Human) from around the phosphorylation site of threonine 2647 (TLTPQT)

  • Positive controlHUVEC cells treated with serum

Properties

Applications

Our Abpromise guarantee covers the use of ab61156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/500 - 1/1000.
ELISA ELISA: 1/5000.

Target

  • FunctionSerine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 2 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
    Contains 3 TPR repeats.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair.
    S-nitrosylated by GAPDH.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P78527 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        DNA dependent protein kinase catalytic subunit antibodyDNA PK catalytic subunit antibodyDNA-dependent protein kinase catalytic subunit antibody
        DNA-PK catalytic subunit antibodyDNA-PKcs antibodyDNAPK antibodyDNAPK catalytic subunit antibodyDNPK 1 antibodyDNPK1 antibodyHyper radiosensitivity of murine scid mutation, complementing 1 antibodyHYRC 1 antibodyHYRC antibodyHYRC1 antibodyp350 antibodyp460 antibodyPKRDC antibodyPRKDC antibodyPRKDC_HUMAN antibodyProtein Kinase DNA Activated Catalytic Polypeptide antibodyXRCC 7 antibodyXRCC7 antibody
      see all

    Anti-DNA PKcs antibody images

    • Immunocytochemistry/ Immunofluorescence - DNA PKcs antibody (ab61156)
      Immunocytochemistry/ Immunofluorescence - DNA PKcs antibody (ab61156)
      Immunofluorescence analysis of HUVEC cells treated with serum (20%, 30 mins) labelled with ab61156 at 1/500 dilution.

    References for Anti-DNA PKcs antibody (ab61156)

    ab61156 has not yet been referenced specifically in any publications.

    Publishing research using ab61156 ? Please let us know so that we can cite the reference in this datasheet

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