This antibody detects a >350 kDa protein, corresponding to the apparent molecular mass of DNA-PKcs. This antibody also detects an ~180 kDa band,
believed to be a processed form of the catalytic subunit.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately >350 and 180 kDa (predicted molecular weight: 470 kDa). (ECL detection).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
Phosphorylated upon DNA damage, probably by ATM or ATR. Autophosphorylated on Thr-2609, Thr-2638 and Thr-2647. Thr-2609 is a DNA damage-inducible phosphorylation site (inducible with ionizing radiation, IR). Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair. S-nitrosylated by GAPDH.
DNA dependent protein kinase catalytic subunit antibody
DNA PK catalytic subunit antibody
DNA-dependent protein kinase catalytic subunit antibody
DNA-PK catalytic subunit antibody
DNAPK catalytic subunit antibody
DNPK 1 antibody
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Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody
HYRC 1 antibody
Protein Kinase DNA Activated Catalytic Polypeptide antibody
XRCC 7 antibody
Western blot - DNA PKcs antibody (ab69527)Image from Ju J et al, J Biol Chem. 2010 Dec 24;285(52):41152-60. Epub 2010 Oct 21, Fig 1. DOI 10.1074/jbc.M110.158352
M059K cells were treated with different doses of DNA-PKcs siRNA; 48 h later, protein extracts were prepared and subjected to immunoblot analysis with ab69527 or an antibody to GAPDH.
This product has been referenced in:
Castellanos-Martín A et al. Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach. Genome Biol16:40 (2015).
Read more (PubMed: 25853295) »
Ju J et al. Phosphorylation of p50 NF-kappaB at a single serine residue by DNA-dependent protein kinase is critical for VCAM-1 expression upon TNF treatment. J Biol Chem285:41152-60 (2010).
Read more (PubMed: 20966071) »