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Synthetic peptide corresponding to a region between residue 4075 and the C-terminus (residue 4128) of human DNA PKcs. (NP_008835.5)
Our Abpromise guarantee covers the use of ab70250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab70250 staining DNA PKCs in T98G Human brain glioblastoma by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 10 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. A CF568-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab70250 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"