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DNA Polymerase beta protein (Tagged-His Tag) (ab91943)

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Overview

  • Product nameDNA Polymerase beta protein (Tagged-His Tag)See all DNA Polymerase beta proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 143-335 (193 amino acids) of Human DNA Polymerase beta with an N terminal His tag. Predicted MWt: 23 kDa; UniProt ID: P06746.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 67 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFR RGAESSGDMD VLLTHPSFTSESTKQPKLLHQVVEQLQK VHFITDTLSKGETKFMGVCQLP SKNDEKEYPHRRIDIR LIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFT INEY TIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91943 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DNA directed DNA polymerase betaDNA pol betaDNA polymerase beta
        DNA polymerase beta subunitDPOLB_HUMANMGC125976Pol BPol betaPolBPolymerase (DNA directed) beta
      see all
  • FunctionRepair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • Sequence similaritiesBelongs to the DNA polymerase type-X family.
  • DomainResidues 239-252 form a flexible loop which appears to affect the polymerase fidelity.
  • Post-translational
    modifications    Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P06746 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    DNA Polymerase beta protein (Tagged-His Tag) images

    • SDS-PAGE - DNA Polymerase beta protein (Tagged-His Tag) (ab91943)
      SDS-PAGE - DNA Polymerase beta protein (Tagged-His Tag) (ab91943)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for DNA Polymerase beta protein (Tagged-His Tag) (ab91943)

    ab91943 has not yet been referenced specifically in any publications.

    Publishing research using ab91943 ? Please let us know so that we can cite the reference in this datasheet

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