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Anti-DNA Polymerase delta, catalytic subunit antibody [607] (ab10362)

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Overview

Product name

Anti-DNA Polymerase delta, catalytic subunit antibody [607]
See all DNA Polymerase delta, catalytic subunit products (6) ...

Description

Mouse monoclonal [607] to DNA Polymerase delta, catalytic subunit

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Recombinant protein, corresponding to amino acids 1-140 of the large subunit of Human DNA Polymerase delta.

Positive control

HeLa whole cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Antibody purified from hybridoma cell culture supernatant by protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE.

Clonality

Monoclonal

Clone number

607

Myeloma

NS1

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Polymerases

  • Western blot - DNA Polymerase delta, catalytic subunit antibody [607] (ab10362)Western blot - DNA Polymerase delta, catalytic subunit antibody [607] (ab10362) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP28340 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CDC2 antibody
  • CDC2 homolog antibody
  • DNA directed DNA polymerase delta 1 antibody
  • DNA directed polymerase delta 1 antibody
  • DNA pol delta 1 antibody
  • DNA polymerase delta catalytic subunit antibody
  • DNA polymerase subunit delta p125 antibody
  • DPOD1_HUMAN antibody
  • POLD antibody
  • POLD 1 antibody
  • POLD1 antibody
  • Polymerase (DNA directed) delta 1 catalytic subunit 125kDa antibody
  • Polymerase DNA directed delta 1 catalytic subunit 125kD antibody
see all

Database links

Anti-DNA Polymerase delta, catalytic subunit antibody [607] images:

  Western blot - DNA Polymerase delta, catalytic subunit antibody [607] (ab10362)

Western blot - DNA Polymerase delta, catalytic subunit antibody [607] (ab10362)



Predicted band size : 130 kDa


Detection of human DNA polymerase delta by using anti-pol delta monoclonal antibody [607] (ab10362) in HeLa whole cell extract.

References for Anti-DNA Polymerase delta, catalytic subunit antibody [607] (ab10362)

This product has been referenced in:

  • Kawabata Tet al. A reduction of licensed origins reveals strain-specific replication dynamics in mice. Mamm Genome 22:506-17 (2011). WB; Mouse.Read more (PubMed: 21611832) »
  • Akbari Met al. Extracts of proliferating and non-proliferating human cells display different base excision pathways and repair fidelity. DNA Repair (Amst) 8:834-43 (2009). WB; Human.Read more (PubMed: 19442590) »

See all 4 publications for this product

Publishing research using ab10362? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"