Anti-DNA Polymerase epsilon antibody (ab105454)

Overview

• Product nameAnti-DNA Polymerase epsilon antibodySee all DNA Polymerase epsilon primary antibodies ...
• Description
  Rabbit polyclonal to DNA Polymerase epsilon
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Zebrafish, Macaque Monkey, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human DNA Polymerase epsilon.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in the following Human tissue lysates: Heart; Brain; Placenta; Testis; Skeletal muscle.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • DNA Polymerases

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • Other

Applications

Target

• FunctionForms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
• Tissue specificityExpressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q9NRF9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 417275 Chicken
  • Entrez Gene: 510678 Cow
  • Entrez Gene: 54107 Human
  • Entrez Gene: 59001 Mouse
  • Entrez Gene: 100362333 Rat
  • Entrez Gene: 298098 Rat
  • Omim: 607267 Human
  • SwissProt: Q3SZN5 Cow

  • SwissProt: Q9NRF9 Human
  • SwissProt: Q9JKP7 Mouse
  • SwissProt: Q642A5 Rat
  • Unigene: 108112 Human
  • Unigene: 27444 Mouse
  • Unigene: 3290 Rat

  see all

• Alternative names
  Arsenic transactivated protein antibodyArsenic-transactivated protein antibodyASTP antibody

  CHARAC 17 antibodyCHARAC17 antibodyCHRAC 17 antibodyCHRAC-17 antibodyCHRAC17 antibodyChromatin Accessibility Complex 17 antibodyChromatin accessibility complex 17 kDa protein antibodyDNA Polymerase Epsilon p17 Subunit antibodyDNA polymerase epsilon subunit 3 antibodyDNA polymerase epsilon subunit p17 antibodyDNA polymerase II subunit 3 antibodyDPOE3_HUMAN antibodyHistone fold protein CHRAC17 antibodyHuCHRAC 17 antibodyHuCHRAC17 antibodyp17 antibodyp17 subunit antibodyPOL E3 antibodyPOLE 3 antibodyPOLE3 antibodyPolymerase (DNA directed) epsilon 3 antibodyYBL 1 antibodyYBL1 antibody

  see all

Anti-DNA Polymerase epsilon antibody images

• 

  Western blot - Anti-DNA Polymerase epsilon antibody (ab105454)
  All lanes : Anti-DNA Polymerase epsilon antibody (ab105454) at 1 µg/ml
  
  Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
  Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 3 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
  Lane 4 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
  Lane 5 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 16 kDa
  Observed band size : 16 kDa
  Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 3 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-DNA Polymerase epsilon antibody (ab105454)
  ICC/IF image of ab105454 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105454, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 1µg/ml.