Anti-CHRAC-17 antibody (ab88521)
Key features and details
- Mouse polyclonal to CHRAC-17
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CHRAC-17 antibody
See all CHRAC-17 primary antibodies -
Description
Mouse polyclonal to CHRAC-17 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human CHRAC-17. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. AAH03166.1
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Positive control
- Human liver tissue lysate. CHRAC-17 transfected 293T cell lysate. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. -
Tissue specificity
Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 54107 Human
- Omim: 607267 Human
- SwissProt: Q9NRF9 Human
- Unigene: 108112 Human
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Alternative names
- Arsenic transactivated protein antibody
- Arsenic-transactivated protein antibody
- ASTP antibody
see all
Images
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Anti-CHRAC-17 antibody (ab88521) at 1 µg/ml + Human liver tissue lysate at 50 µg
Predicted band size: 17 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CHRAC-17 antibody (ab88521) at 1 µg/ml
Lane 1 : CHRAC-17 transfected HEK-293T cell lysate
Lane 2 : Non-transfected HEK-293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 17 kDa
Observed band size: 17 kDa -
ab88521, at 10 µg/ml, staining CHRAC-17 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab88521 has not yet been referenced specifically in any publications.