Anti-DNA Polymerase gamma antibody (ab97661)

Overview

  • Product nameAnti-DNA Polymerase gamma antibodySee all DNA Polymerase gamma primary antibodies ...
  • Description
    Rabbit polyclonal to DNA Polymerase gamma
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to a sequence within amino acids 779-1201 of Human DNA Polymerase gamma (NP_002684).

  • Positive control
    • 293T, HeLa, HepG2, MCF7 and MOLT4 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab97661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Predicted molecular weight: 140 kDa.

Target

  • FunctionInvolved in the replication of mitochondrial DNA.
  • Involvement in diseaseDefects in POLG are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 1 (PEOA1) [MIM:157640]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
    Defects in POLG are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal recessive (PEOB) [MIM:258450]. PEOB is a severe form of progressive external ophthalmoplegia. It is clinically more heterogeneous than the autosomal dominant forms. Can be more severe.
    Defects in POLG are a cause of sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO) [MIM:607459]. SANDO is a clinically heterogeneous systemic disorder with variable features resulting from mitochondrial dysfunction. It shares phenotypic characteristics with autosomal recessive progressive external ophthalmoplegia and mitochondrial neurogastrointestinal encephalopathy syndrome. The clinical triad of symptoms consists of sensory ataxic, neuropathy, dysarthria, and ophthalmoparesis.
    Defects in POLG are a cause of Alpers-Huttenlocher syndrome (AHS) [MIM:203700]; also called Alpers diffuse degeneration of cerebral gray matter with hepatic cirrhosis. AHS is an autosomal recessive hepatocerebral syndrome. The typical course of AHS includes severe developmental delay, intractable seizures, liver failure, and death in childhood. Refractory seizures, cortical blindness, progressive liver dysfunction, and acute liver failure after exposure to valproic acid are considered diagnostic features. The neuropathological hallmarks of AHS are neuronal loss, spongiform degeneration, and astrocytosis of the visual cortex. Liver biopsy results show steatosis, often progressing to cirrhosis.
    Defects in POLG are a cause of mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE) [MIM:603041]; also known as myoneurogastrointestinal encephalomyopathy. MNGIE is an autosomal recessive disease associated with multiple deletions of skeletal muscle mitochondrial DNA (MtDNA). It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, thin body habitus, peripheral neuropathy, and myopathy.
    Defects in POLG are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
  • Sequence similaritiesBelongs to the DNA polymerase type-A family.
  • Cellular localizationMitochondrion.
  • Target information above from: UniProt accession P54098 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DNA directed DNA polymerase gamma antibody
    • DNA polymerase subunit gamma 1 antibody
    • DNA polymerase subunit gamma-1 antibody
    • DPOG1_HUMAN antibody
    • MDP 1 antibody
    • MDP1 antibody
    • Mitochondrial DNA polymerase catalytic subunit antibody
    • Mitochondrial DNA polymerase gamma antibody
    • PEO antibody
    • POLG 1 antibody
    • POLG A antibody
    • PolG alpha antibody
    • POLG antibody
    • PolG-alpha antibody
    • POLG1 antibody
    • POLGA antibody
    • Polymerase (DNA directed) gamma antibody
    • SANDO antibody
    • SCAE antibody
    see all

Anti-DNA Polymerase gamma antibody images

  • Anti-DNA Polymerase gamma antibody (ab97661) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size : 140 kDa

References for Anti-DNA Polymerase gamma antibody (ab97661)

ab97661 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting the review of ab97661. I am sorry to see that the results with this antibody were unsatisfactory. We do guarantee all of our products to work as stated on the datasheet for up to 6 months after purchase. Since you have ...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (Tumor cells)
Loading amount 30 µg
Specification Tumor cells
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Prof. Yongliang Zhao

Verified customer

Submitted Nov 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"