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Western blot - DNA polymerase delta p50 antibody [2762C3a] (ab53741)
![Western blot - DNA polymerase delta p50 antibody [2762C3a] (ab53741)](/ps/datasheet/images/pol.jpg)
(enlarge)
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Product Name
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DNA polymerase delta p50 antibody [2762C3a]
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See all DNA polymerase delta p50 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [2762C3a] to DNA polymerase delta p50
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Immunogen
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Recombinant fragment (Human) from near the N terminus.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Dot, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Dot: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Polymerases
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Relevance
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DNA polymerase delta is composed of 2 polypeptides present in equimolar ratio. The 125-kD subunit, POLD1, contains both the polymerase and the 3-prime-to-5-prime exonuclease activities, whereas the function of the 48-kD subunit is unknown. In addition to DNA replication, DNA polymerase delta is also involved in nucleotide excision repair of DNA, and PCNA, an accessory protein for DNA polymerase delta, is essential for both processes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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2762C3a
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DNA polymerase delta p50 antibody [2762C3a] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DNA polymerase delta p50 antibody [2762C3a] images, references, products related to ab53741 and other tools.
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DNA polymerase delta p50 antibody [2762C3a] images:
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 Western blot - DNA polymerase delta p50 antibody [2762C3a] (ab53741)
DNA polymerase delta p50 antibody [2762C3a] (ab53741) + immunizing recombinant protein
Predicted band size : 52 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
The size of the band does not correspond to the predicted protein molecular weight as the immunizing recombinant protein was used for the test lane
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Search PubMed (MEDLINE) for references to DNA polymerase delta p50
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DNA polymerase delta p50 antibody [2762C3a] - more information
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Customer reviews (feedback) regarding DNA polymerase delta p50 antibody [2762C3a] |
Customer FAQs regarding DNA polymerase delta p50 antibody [2762C3a] |
Protocols for DNA polymerase delta p50 antibody [2762C3a] |
Price and availability of products related to DNA polymerase delta p50 antibody [2762C3a] |
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DNA polymerase delta p50 antibody [2762C3a] - related products:
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DNA polymerase delta p50 antibody [2762C3a] - other tools:
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Contact Abcam with a Technical Enquiry about DNA polymerase delta p50 antibody [2762C3a] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - DNA polymerase delta p50 antibody [2762C3a] (ab53741)](/index.html?datasheet=53741#pol.jpg)
