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Western blot - DNA polymerase delta p50 antibody (ab38338)
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Immunocytochemistry/ Immunofluorescence - DNA polymerase delta p50 antibody (ab38338)
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Product Name
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DNA polymerase delta p50 antibody
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See all DNA polymerase delta p50 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DNA polymerase delta p50
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human DNA polymerase delta p50.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Cow, Mcq
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab38338 gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line),
A431 (Human epithelial carcinoma cell line),
HEK 293 (Human embryonic kidney cell line),
HepG2 (Human hepatocellular liver carcinoma cell line),
MCF-7 (Human breast adenocarcinoma cell line).
and the following Nuclear lysate:
HeLa (Human epithelial carcinoma cell line).
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Polymerases
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Relevance
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DNA polymerase delta is composed of 2 polypeptides present in equimolar ratio. The 125-kD subunit, POLD1, contains both the polymerase and the 3-prime-to-5-prime exonuclease activities, whereas the function of the 48-kD subunit is unknown. In addition to DNA replication, DNA polymerase delta is also involved in nucleotide excision repair of DNA, and PCNA, an accessory protein for DNA polymerase delta, is essential for both processes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DNA polymerase delta p50 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DNA polymerase delta p50 antibody images, references, products related to ab38338 and other tools.
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DNA polymerase delta p50 antibody images:
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 Immunocytochemistry/ Immunofluorescence - DNA polymerase delta p50 antibody (ab38338)
ICC/IF image of ab38338 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38338, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to DNA polymerase delta p50
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DNA polymerase delta p50 antibody - more information
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