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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Polymerases
Overview
Product name
Anti-DNA polymerase delta p50 antibody
See all DNA polymerase delta p50 products (2) ...
Description
Rabbit polyclonal to DNA polymerase delta p50
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Macaque Monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human DNA polymerase delta p50.
Positive control
ab38338 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK 293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line), MCF-7 (Human breast adenocarcinoma cell line). and the following Nuclear lysate: HeLa (Human epithelial carcinoma cell line).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> DNA Polymerases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
Target
Function
The function of the small subunit is not yet clear.
Sequence similarities
Belongs to the DNA polymerase delta/II small subunit family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP49005
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA polymerase delta subunit 2 antibody
- DNA polymerase delta subunit p50 antibody
- DNA polymerase subunit delta 2 antibody
see all
Database links
- Entrez Gene: 281991 Cow
- Entrez Gene: 5425 Human
- Entrez Gene: 18972 Mouse
- Entrez Gene: 289758 Rat
- Omim: 600815 Human
- SwissProt: P49004 Cow
- SwissProt: P49005 Human
- SwissProt: O35654 Mouse
see all
Anti-DNA polymerase delta p50 antibody images:
Western blot - DNA polymerase delta p50 antibody (ab38338)
All lanes : Anti-DNA polymerase delta p50 antibody (ab38338) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 5 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 51 kDa
Observed band size : 51 kDa
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - DNA polymerase delta p50 antibody (ab38338)
ICC/IF image of ab38338 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38338, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-DNA polymerase delta p50 antibody (ab38338)
ab38338 has not yet been referenced specifically in any publications.
Publishing research using ab38338? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"