Overview

  • Product nameAnti-DNAJC3 antibodySee all DNAJC3 primary antibodies ...
  • Description
    Rabbit polyclonal to DNAJC3
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human DNAJC3.

    (Peptide available as ab72884.)

  • Positive control
    • This antibody gives a positive signal in the following lysates: Rat Thymus Tissue TE 671 Whole Cell Y79 Whole Cell Hela - Staurosporine Treated (24hr, 500nM) Jurkat - Staurosporine Treated (24hr, 500nM).

Properties

Applications

Our Abpromise guarantee covers the use of ab72885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human DNAJC3 peptide (ab72884).

Target

  • FunctionInvolved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
  • Tissue specificityWidely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
  • Sequence similaritiesContains 1 J domain.
    Contains 9 TPR repeats.
  • DomainThe J domain mediates interaction with HSPA8.
  • Cellular localizationEndoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • DnaJ (Hsp40) homolog subfamily C member 3 antibody
    • DnaJ homolog subfamily C member 3 antibody
    • DNAJC3 antibody
    • DNJC3_HUMAN antibody
    • double-stranded RNA-activated protein kinase inhibitor antibody
    • Endoplasmic reticulum DnaJ protein 6 antibody
    • ERdj6 antibody
    • HP58 antibody
    • Interferon induced, double stranded RNA activated protein kinase inhibitor antibody
    • Interferon-induced antibody
    • P58 antibody
    • p58 ipk antibody
    • P58IPK antibody
    • PRKRI antibody
    • Protein kinase inhibitor of 58 kDa antibody
    • Protein kinase inhibitor p58 antibody
    • Protein kinase interferon inducible double stranded RNA dependent inhibitor antibody
    see all

Anti-DNAJC3 antibody images

  • All lanes : Anti-DNAJC3 antibody (ab72885) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 5 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab72885 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72885, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, HepG2, Hek293, MCF-7 cells at 1µg/ml.

References for Anti-DNAJC3 antibody (ab72885)

ab72885 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab72885.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"