Anti-DOCK1 antibody (ab76927)

Overview

• Product nameAnti-DOCK1 antibodySee all DOCK1 primary antibodies ...
• Description
  Rabbit polyclonal to DOCK1
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residues 1815 and 1865 of human DOCK1 (NP_001371.1)

• Positive controlHeLa, 293T and NIH3T3 whole cell lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab76927 was affinity purified using an epitope specific to DOCK1 immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • Small G Proteins
  • Regulators

  • Cell Biology
  • Apoptosis
  • Phagocytosis

Applications

Target

• FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
• Tissue specificityHighly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
• Sequence similaritiesBelongs to the DOCK family.
  Contains 1 DHR-1 (CZH-1) domain.
  Contains 1 DHR-2 (CZH-2) domain.
  Contains 1 SH3 domain.
• DomainThe DHR-2 domain is necessary and sufficient for the GEF activity.
• Cellular localizationCytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.

Target information above from: UniProt accession Q14185 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1793 Human
  • Entrez Gene: 330662 Mouse
  • Omim: 601403 Human
  • SwissProt: Q14185 Human
  • SwissProt: Q8BUR4 Mouse
  • Unigene: 645702 Human
  • Unigene: 159195 Human
  • Unigene: 436572 Mouse

• Alternative names
  180 kDa protein downstream of CRK antibodyCed5 antibodyDedicator of Cytokinesis 1 antibody

  Dedicator of cytokinesis protein 1 antibodyDOCK 1 antibodyDOCK 180 antibodyDOCK1 antibodyDOCK1_HUMAN antibodyDOCK180 antibody

  see all

Anti-DOCK1 antibody images

• 

  Western blot - DOCK1 antibody (ab76927)
  All lanes : Anti-DOCK1 antibody (ab76927) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  Lane 5 : NIH3T3 whole cell lysate at 50 µg
  
  
  Predicted band size : 215 kDa
  Observed band size : 215 kDa
  
  
  Exposure time : 3 minutes
• 

  Immunoprecipitation - DOCK1 antibody (ab76927)
  Detection of DOCK1 by Western Blot of Immunprecipitate.
  ab76927, at 1µg/ml, staining DOCK1 in HeLa whole cell lysate immunoprecipitated using ab76927 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
  Detection: Chemiluminescence with exposure time of 3 seconds.