Anti-ACG antibody (ab190270)
Key features and details
- Rabbit polyclonal to ACG
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACG antibody
See all ACG primary antibodies -
Description
Rabbit polyclonal to ACG -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human ACG aa 250-400.
Database link: Q5JSL3 -
Positive control
- IHC-P: Human placenta, kidney and testis tissues. ICC: RH-30 whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. -
Sequence similarities
Belongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
Contains 1 PH domain. -
Domain
The DHR-2 domain is necessary for the GEF activity. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 139818 Human
- Entrez Gene: 75974 Mouse
- Entrez Gene: 313438 Rat
- Omim: 300681 Human
- SwissProt: Q5JSL3 Human
- SwissProt: A2AF47 Mouse
- Unigene: 368203 Human
- Unigene: 32873 Mouse
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Alternative names
- ACG antibody
- Activated Cdc42-associated guanine nucleotide exchange factor antibody
- Dedicator of cytokinesis protein 11 antibody
see all
Images
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Immunohistochemical analysis of human placenta tissue labeling ACG in the nuclear membrane of trophoblastic cells with ab190270 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of RH-30 (Human bone marrow metastasis cell line) whole cells labeling ACG in the nucleoli and nuclear membrane with ab190270 at 2 µg/ml.
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Immunohistochemical analysis of human kidney tissue labeling ACG in the nuclear membrane of cells in tubules with ab190270 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human testis tissue labeling ACG in the nuclear membrane of cells in seminiferous ducts with ab190270 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human liver tissue labeling ACG with ab190270 at a 1/50 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190270 has not yet been referenced specifically in any publications.