Anti-DOK2 antibody (ab131488)

Overview

• Product nameAnti-DOK2 antibodySee all DOK2 primary antibodies ...
• Description
  Rabbit polyclonal to DOK2
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH, surrounding internal sequence amino acids 297-301 (G-E-Y-A-V) of Human DOK2 (NP_003965.2).

• Positive controlJurkat cell extract; HeLa cells; Human breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: PBS without Mg²⁺ and Ca²⁺
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab131488 was purified by affinity chromatography using epitope specific peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Other

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Tyrosine kinases
  • Other

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Signal Transduction
  • Adapters
  • Cytoplasmic

Applications

Target

• FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
• Tissue specificityHighly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
• Sequence similaritiesBelongs to the DOK family. Type A subfamily.
  Contains 1 IRS-type PTB domain.
  Contains 1 PH domain.
• DomainPTB domain mediates receptor interaction.
• Post-translational
  modificationsOn immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.

Target information above from: UniProt accession O60496 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9046 Human
  • Omim: 604997 Human
  • SwissProt: O60496 Human
  • Unigene: 71215 Human

• Alternative names
  Docking protein 2 56kDa antibodyDocking protein 2 antibodyDOK 2 antibody

  DOK R antibodyDOK2 antibodyDOK2_HUMAN antibodyDownstream of tyrosine kinase 2 antibodyOTTHUMP00000161710 antibodyOTTHUMP00000224923 antibodyp56(dok 2) antibodyp56(dok-2) antibodyp56dok 2 antibodyp56DOK antibodyp56dok2 antibody

  see all

Anti-DOK2 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-DOK2 antibody (ab131488)
  Immunofluorescent analysis of methanol-fixed HeLa cells labelling DOK2 with ab131488 at 1/100 dilution.
• 

  Western blot - Anti-DOK2 antibody (ab131488)
  Anti-DOK2 antibody (ab131488) at 1/500 dilution + Jurkat cell extract
  
  Predicted band size : 45 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DOK2 antibody (ab131488)
  Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labelling DOK2 with ab131488 at 1/50 dilution. The image on the right is treated with the synthesized peptide.