Anti-DOK3 antibody (ab89327)

Overview

• Product nameAnti-DOK3 antibodySee all DOK3 primary antibodies ...
• Description
  Mouse polyclonal to DOK3
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length human DOK3 protein (AAH04564.2; Q7L591-3).

• Positive controlHuman liver tissue lysate, DOK3 transfected 293T cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • MAPK pathway

  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • Small G Proteins
  • Ras Family

Applications

Target

• FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
• Tissue specificityExpressed in spleen.
• Sequence similaritiesBelongs to the DOK family. Type A subfamily.
  Contains 1 IRS-type PTB domain.
  Contains 1 PH domain.
• DomainPTB domain mediates receptor interaction.
• Post-translational
  modificationsConstitutively tyrosine-phosphorylated.
  On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
• Cellular localizationCytoplasm. Cell membrane.

Target information above from: UniProt accession Q7L591 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 79930 Human
  • Entrez Gene: 79930 Human
  • Omim: 611435 Human
  • SwissProt: Q7L591 Human
  • SwissProt: Q7L591 Human
  • Unigene: 720849 Human

• Alternative names
  Docking protein 3 antibodyDOK 3 antibodyDOK Like Protein antibody

  Dok3 antibodyDOK3_HUMAN antibodyDOKL Pending antibodyDownstream of tyrosine kinase 3 antibodyp62 DOK Like Protein antibody

  see all

Anti-DOK3 antibody images

• 

  Western blot - DOK3 antibody (ab89327)
  Anti-DOK3 antibody (ab89327) at 1 µg/ml + human liver tissue lysate at 50 µg
  
  Predicted band size : 36 kDa
  Observed band size : ~58 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - DOK3 antibody (ab89327)
  All lanes : Anti-DOK3 antibody (ab89327) at 1 µg/ml
  
  Lane 1 : DOK3 transfected 293T cell lysate
  Lane 2 : Non transfected lysate
  
  Lysates/proteins at 25 µg per lane.
  
  
  Predicted band size : 36 kDa
  Observed band size : 36 kDa