Anti-DOK5 antibody - Aminoterminal end (ab71308)

Overview

• Product nameAnti-DOK5 antibody - Aminoterminal endSee all DOK5 primary antibodies ...
• Description
  Rabbit polyclonal to DOK5 - Aminoterminal end
• Tested applicationsWB, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Positive controlMouse liver tissue lysate, human cancer tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Adapters
  • Transmembrane

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

Applications

Target

• FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.
• Tissue specificityHighest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.
• Sequence similaritiesBelongs to the DOK family. Type B subfamily.
  Contains 1 IRS-type PTB domain.
  Contains 1 PH domain.
• DomainPTB domain mediates receptor interaction.
• Post-translational
  modificationsPhosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.

Target information above from: UniProt accession Q9P104 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 55816 Human
  • Entrez Gene: 76829 Mouse
  • Omim: 608334 Human
  • SwissProt: Q9P104 Human
  • SwissProt: Q91ZM9 Mouse
  • Unigene: 656582 Human
  • Unigene: 41633 Mouse

• Alternative names
  chromosome 20 open reading frame 180 antibodyDocking protein 5 antibodyDOK 5 antibody

  DOK5 antibodyDOK5 protein antibodyDOK5_HUMAN antibodydownstream of tyrosine kinase 5 antibodyInsulin receptor substrate 6 antibodyIRS-6 antibodyIRS6 antibody

  see all

Anti-DOK5 antibody - Aminoterminal end images

• 

  Western blot - DOK5 antibody - Aminoterminal end (ab71308)
  Anti-DOK5 antibody - Aminoterminal end (ab71308) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg
  
  Predicted band size : 35 kDa
  Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 100 kDa,43 kDa,80 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DOK5 antibody - Aminoterminal end (ab71308)
  Formalin-fixed and paraffin-embedded human breast cancer tissue reacting with ab71308, peroxidase-conjugated secondary used, followed by AEC staining.