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Product Name
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DOPA Decarboxylase antibody [DDC-109]
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See all DOPA Decarboxylase antibodies (8)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [DDC-109] to DOPA Decarboxylase
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Immunogen
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A synthetic human DOPA Decarboxylase N terminal peptide (IEGRQVYPDVEPGYLR) corresponding to amino acids 24-39 conjugated to KLH.
BLAST 'IEGRQVYPDVEPGYLR' with  or 
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Reacts with
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Hu, Rat, Cow, Dog, Gpig, Mk, Rb, Shp
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Predicted to react
(species key)
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Ms, Pig, Zfsh
(Due to sequence homology)
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Specificity
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This antibody is specific to DOPA Decarboxylase
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Tested applications
(see key)
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I-ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
I-ELISA: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 54 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Bovine brain extract
ICC/IF: Brain tissue
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine
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Relevance
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DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme.
In immunocytochemical labeling of brain tissue, the product stains the monoaminergic cells and proximal dendrites, but not cortical fibers.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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DDC-109
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Isotype
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IgG2b
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Purity
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Ascites
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Ascites Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.700 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DOPA Decarboxylase antibody [DDC-109] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to DOPA Decarboxylase
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DOPA Decarboxylase antibody [DDC-109] - more information
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