Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab36155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

Target

  • Function
    Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
  • Pathway
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2.
  • Involvement in disease
    Defects in DDC are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]. AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.
  • Sequence similarities
    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AADC antibody
    • Aromatic L Amino Acid Decarboxylase antibody
    • Aromatic-L-amino-acid decarboxylase antibody
    • DDC antibody
    • DDC_HUMAN antibody
    • DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody
    • DOPA decarboxylase antibody
    see all

Images

  • Anti-DOPA Decarboxylase antibody (ab36155) at 0.03 µg/ml (incubated for 1 hour) + Human Kidney lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 54 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Western blot showing a DOPA decarboxylase band at approximately 50kDa in human kidney lysate using ab36155 at 0.03µg/ml(35µg protein in RIPA buffer). 1 hour primary incubation. Protein detected by chemiluminescence.

References

ab36155 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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