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Western blot - DOPA Decarboxylase antibody (ab37192)
(enlarge)
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Product Name
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DOPA Decarboxylase antibody
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See all DOPA Decarboxylase antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DOPA Decarboxylase
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Immunogen
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Synthetic peptide from N terminal region of Human DOPA Decarboxylase (DDCH), conjugated to keyhole limpet hemocyanin (KLH).
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Reacts with
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Hu, Rat, Cow, Dog, Gpig, Pig, Shp
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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bovine adrenal medulla lysate
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine
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Relevance
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DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, 1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DOPA Decarboxylase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DOPA Decarboxylase antibody images, references, products related to ab37192 and other tools.
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DOPA Decarboxylase antibody images:
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 Western blot - DOPA Decarboxylase antibody (ab37192)
DOPA Decarboxylase antibody (ab37192) at 1/1000 dilution + bovine adrenal medulla lysate
Predicted band size : 54 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Lysate loading concentration: 5µg
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Search PubMed (MEDLINE) for references to DOPA Decarboxylase
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DOPA Decarboxylase antibody - more information
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Customer reviews (feedback) regarding DOPA Decarboxylase antibody |
Customer FAQs regarding DOPA Decarboxylase antibody |
Protocols for DOPA Decarboxylase antibody |
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DOPA Decarboxylase antibody - related products:
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DOPA Decarboxylase antibody - other tools:
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Contact Abcam with a Technical Enquiry about DOPA Decarboxylase antibody |
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