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Immunocytochemistry - DOPA Decarboxylase antibody (ab3905)
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Immunohistochemistry (Frozen sections) - DOPA Decarboxylase antibody (ab3905)
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Product Name
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DOPA Decarboxylase antibody
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See all DOPA Decarboxylase antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to DOPA Decarboxylase
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Immunogen
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SDS denatured, recombinant bovine aromatic L-amino acid decarboxylase expressed in E. coli and purified from inclusion bodies
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Reacts with
(species key)
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Ms, Rat
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Tested applications
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ICC, IHC-FoFr, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependant dilution.
IHC-Fr: Used at a dilution of 1/500 overnight incubation on mouse brain slices (see Abreview for further information).
WB: Use at a concentration of 0.1 - 0.5 µg/ml (tested against adrenal, kidney, and caudate nucleus samples).
IHC-FoFr: Use at 1:500
By Western blot, this antibody detects a band of 50 kDa, which corresponds to the predicted molecular weight of DOPA Decarboxylase.
Much lower concentrations, than that suggested for Western blot, should be used in immunocytochemical applications.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine
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Relevance
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DOPA decarboxylase is an enzyme implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Following the hydroxylation of tyrosine to form L dihydroxyphenylalanine (LDOPA), catalyzed by tyrosine hydroxylase, DDC decarboxylates LDOPA to form dopamine. This neurotransmitter is found in different areas of the brain and is particularly abundant in basal ganglia. Dopamine is also produced by DDC in the sympathetic nervous system and is the precursor of the catecholaminergic hormones, noradrenaline and adrenaline in the adrenal medulla. In the nervous system, tryptophan hydroxylase produces 5 OH tryptophan, which is decarboxylated by DDC, giving rise to serotonin. DDC is a homodimeric, pyridoxal phosphate dependent enzyme.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 10% Glycerol, 0.05% Tween 20, PBS, 1mg/ml BSA, 20mM Tris HCl, 10mM HEPES, 0.1% PVP-40, 0.1% NaN3. pH 7.2 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this DOPA Decarboxylase antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for DOPA Decarboxylase antibody images, references, products related to ab3905 and other tools.
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DOPA Decarboxylase antibody images:
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 Immunocytochemistry - DOPA Decarboxylase antibody (ab3905)
ab3905 at 1/2000 staining mouse brain cells by ICC. The cells were paraformaldehyde fixed and permeabilized, then blocked with serum and incubated with the antibody for 16 hours at 4°C. A biotinylated goat anti-rabbit IgG was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Frozen sections) - DOPA Decarboxylase antibody (ab3905)
ab3905 at a 1/500 dilution staining DOPA Decarboxylase in rat brain tissue sections by Immunohistochemistry (frozen sections) incubated for 16 hours at 25°C. PFA fixed. Blocked with 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488 (green). This image is courtesy of an anonymous abreview.
See Abreview
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References for DOPA Decarboxylase antibody (ab3905)
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This product has been used in: (two most recent references)
Hoerder-Suabedissen A et al. Novel markers reveal subpopulations of subplate neurons in the murine cerebral cortex. Cereb Cortex 19:1738-50 (2009). PubMed: 19008461
Jiang H & Xiang M Subtype specification of GABAergic amacrine cells by the orphan nuclear receptor Nr4a2/Nurr1. J Neurosci 29:10449-59 (2009). Mouse. PubMed: 19692620
See all 5 publication references for this product.
If you publish research using ab3905 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to DOPA Decarboxylase
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