![Western blot - DPM1 antibody [5C5A7] (ab113686) image](#DPM1-Primary-antibodies-ab113686-2.jpg)
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Product name
Anti-DPM1 antibody [5C5A7]
See all DPM1 products (4) ...
Description
Mouse monoclonal [5C5A7] to DPM1
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant fragment
Positive control
yeast lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Clonality
Monoclonal
Clone number
5C5A7
Isotype
IgG1
Light chain type
kappa
Research areas
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
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Western blot - DPM1 antibody [5C5A7] (ab113686)
![Western blot - DPM1 antibody [5C5A7] (ab113686) image](/ps/datasheet/images/113/ab113686/DPM1-Primary-antibodies-ab113686-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab113686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 4 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Target
Function
Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.
Pathway
Protein modification; protein glycosylation.
Involvement in disease
Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E) [MIM:608799]. CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features.
Sequence similarities
Belongs to the glycosyltransferase 2 family.
Cellular localization
Endoplasmic reticulum.
Target information above from: UniProt accessionO60762
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDGIE antibody
- dolichol monophosphate mannose synthase antibody
- Dolichol phosphate mannose synthase antibody
see all
Database links
- Entrez Gene: 856313 Saccharomyces cerevisiae
- SwissProt: P14020 Saccharomyces cerevisiae
Anti-DPM1 antibody [5C5A7] images:
Western blot - DPM1 antibody [5C5A7] (ab113686)
Anti-DPM1 antibody [5C5A7] (ab113686) + Yeast whole cell lysate at 15 µg
Predicted band size : 30 kDa
References for Anti-DPM1 antibody [5C5A7] (ab113686)
ab113686 has not yet been referenced specifically in any publications.
Publishing research using ab113686? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"