Anti-DPP3 antibody (ab97437)
Key features and details
- Rabbit polyclonal to DPP3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DPP3 antibody
See all DPP3 primary antibodies -
Description
Rabbit polyclonal to DPP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 138 and 435 of DPP3 (NP_005691).
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Positive control
- WB: 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; IHC: AGS xenograft. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF | (1) |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Cleaves Arg-Arg-beta-naphthylamide. -
Sequence similarities
Belongs to the peptidase M49 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10072 Human
- Entrez Gene: 75221 Mouse
- Entrez Gene: 114591 Rat
- Omim: 606818 Human
- SwissProt: Q9NY33 Human
- SwissProt: Q99KK7 Mouse
- SwissProt: O55096 Rat
- Unigene: 502914 Human
see all -
Alternative names
- Dipeptidyl aminopeptidase III antibody
- Dipeptidyl arylamidase III antibody
- Dipeptidyl peptidase 3 antibody
see all
Images
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Anti-DPP3 antibody (ab97437) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 83 kDa -
Top: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97437 at 1/200 dilution. Bottom: image merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ab97437 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab97437 has been referenced in 1 publication.
- Hast BE et al. Cancer-derived mutations in KEAP1 impair NRF2 degradation but not ubiquitination. Cancer Res 74:808-17 (2014). Human . PubMed: 24322982