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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Overview
Product name
Anti-DPP6 antibody - Spacer region
See all DPP6 products (3) ...
Description
Rabbit polyclonal to DPP6 - Spacer region
Specificity
This antibody will recognize the three longest forms of DPP6 but not the 353 amino acid form. It will not recognize the other family members.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide based on the spacer region of human DPP6.
(Peptide available as ab44768.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
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Western blot - DPP6 antibody (ab41810)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 98 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
DPP6 (DPPX, also known as potassium channel accelerating factor Dppx) is a Type-II serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP6 has an Asp instead of Ser in the catalytic site. DPP6 is a member of a broader family of dipeptidyl peptidases including DPP4, FAP/Seprase, DPP2, DPP8, DPP9, DPP10, which have differing substrate specificity and tissue localizations. The surface-bound DPP6 is a homodimer, and cleavage of in the stalk region releases a shed form of DPP6. The shed is the form found in serum. DPP6 has been found in highest abundance in the brain, but also in the kidney, liver and lung.
Cellular localization
Cell Membrane; single-pass type II membrane protein
Alternative names
- Dipeptidyl aminopeptidase like protein 6 antibody
- Dipeptidyl aminopeptidase related protein antibody
- Dipeptidyl peptidase 6 antibody
see all
Database links
- Entrez Gene: 1804 Human
- Entrez Gene: 13483 Mouse
- Entrez Gene: 29272 Rat
- Omim: 126141 Human
- SwissProt: P42658 Human
- SwissProt: Q8IYG9 Human
- SwissProt: Q3TY19 Mouse
- SwissProt: Q5U4C2 Mouse
see all
Anti-DPP6 antibody - Spacer region images:
Western blot - DPP6 antibody (ab41810)
All lanes : Anti-DPP6 antibody - Spacer region (ab41810) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : DU145/IL1-beta Lysate
Predicted band size : 98 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 115 kDa (possible dimer),37 kDa (possible truncated form).
Protocols
References for Anti-DPP6 antibody - Spacer region (ab41810)
ab41810 has not yet been referenced specifically in any publications.
Publishing research using ab41810? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"