DPP8 peptide (Aminoterminal end) (ab44587)

Product Name 

DPP8 peptide (Aminoterminal end)

Product type 

Proteins and Peptides

Peptide 

Synthetic peptide DPP8.


This peptide was used as an immunogen for ab42075 - DPP8 antibody - Aminoterminal end.

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all DPP8 antibodies

Tested applications 
(see key)

ELISA, WB

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab42075. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs

Relevance 

DPP-8 (Dipeptidyl Peptidase-8) is a serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP-8 is a member of a broader family of dipeptidyl peptidases including DPP-2, DPP-4, FAP/Seprase, DPP-6, DPP-9, DPP-10, which have differing substrate specificity and tissue localizations. DPP-8 is most closely related to DPP-9, with which it shares 58% identical residues. DPP-8 has only 27% identity with DPP-4, but seems to have similar substrate specificity. Although DPP-4 and FAP-a form a surface-bound heterodimer in some cells, and homodimers in others, DPP-8 seems to be a cytoplasmic protein. The original publication determined that DPP-8 was a monomer, active at neutral pH, and later publications suggest DPP-8 is a homodimer. Substrates include glucagon-like peptide-1, glucagon-like peptide-2, neuropeptide-Y, but with different efficacy relative to DPP-4. DPP-8 has been found in highest abundance in the testis and placenta, but is found in most tissues. Several different splice variants of DPP-8 were seen in the different tissues, with some tissue-specific expression. Four different human sequences are reported to date; 898, 882, 847 and 782 amino acids in length. The longest sequence of human DPP-8 has a predicted mass of 103.4 kDa, and pI of 5.42. The 882, 847 and 782 amino acid forms are predicted at 101.4, 97.5 and 89.9 kDa respectively, with pIs of 5.26, 5.55 and 5.07. Another three partial sequences that are alternatively spliced have deletions in the catalytic domain, and may not be catalytically active. Over expression of DPP-8 caused decreased viability in cells, but a catalytically dead mutant of DPP-8 had the same results, thus some of the DPP-8 activity may be non-enzymatic. Two of the splice variants have longer aminotermini (16 AA insert), and this may affect localization in the cell.

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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