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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Overview
Product name
DPP8 peptide (Catalytic domain)
See all DPP8 products (4) ...
Protein description
Synthetic peptide DPP8. (Note: the amino acid sequence is proprietary) This peptide was used as an immunogen for ab42077 - DPP8 antibody - Catalytic domain.
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.001% Tween 20, 30mM HEPES, 2mM EDTA, 150mM Sodium chloride, pH 6.75
Concentration
Concentration information loading...
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab44595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This peptide can be used with studies using ab42077. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
DPP8 (Dipeptidyl Peptidase-8) is a serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP8 is a member of a broader family of dipeptidyl peptidases including DPP2, DPP4, FAP/Seprase, DPP6, DPP9, DPP10, which have differing substrate specificity and tissue localizations. DPP8 is most closely related to DPP9, with which it shares 58% identical residues. DPP8 has only 27% identity with DPP4, but seems to have similar substrate specificity. Although DPP4 and FAPa form a surface-bound heterodimer in some cells, and homodimers in others, DPP8 seems to be a cytoplasmic protein. The original publication determined that DPP8 was a monomer, active at neutral pH, and later publications suggest DPP-8 is a homodimer. Substrates include glucagon-like peptide-1, glucagon-like peptide-2, neuropeptide-Y, but with different efficacy relative to DPP4. DPP8 has been found in highest abundance in the testis and placenta, but is found in most tissues. Several different splice variants of DPP8 were seen in the different tissues, with some tissue-specific expression. Four different human sequences are reported to date; 898, 882, 847 and 782 amino acids in length. The longest sequence of human DPP8 has a predicted mass of 103.4 kDa, and pI of 5.42. The 882, 847 and 782 amino acid forms are predicted at 101.4, 97.5 and 89.9 kDa respectively, with pIs of 5.26, 5.55 and 5.07. Another three partial sequences that are alternatively spliced have deletions in the catalytic domain, and may not be catalytically active. Over expression of DPP8 caused decreased viability in cells, but a catalytically dead mutant of DPP8 had the same results, thus some of the DPP8 activity may be non-enzymatic. Two of the splice variants have longer aminotermini (16 AA insert), and this may affect localization in the cell.
Cellular localization
Cytoplasmic
References for DPP8 peptide (Catalytic domain) (ab44595)
ab44595 has not yet been referenced specifically in any publications.
Publishing research using ab44595? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"