Anti-DPP9 antibody - Aminoterminal end (ab42078)

Western blot - DPP9 antibody - Aminoterminal end (ab42078)

All lanes : Anti-DPP9 antibody - Aminoterminal end (ab42078) at 1/1000 dilution

Lane 1 : Cell line DU 145 treated with SF
Lane 2 : Cell line DU 145 treated with RA
Lane 3 : Cell line DU 145 treated with IL1-beta
Lane 4 : Cell line DU 145 treated with SF
Lane 5 : Cell line DU 145 treated with IL1-beta
Lane 6 : Cell line DU 145 treated with Progesterone
Lane 7 : Cell line DU 145 treated with Testosterone


Predicted band size : 98 kDa
Observed band size : 98 kDa

Western blot - DPP9 antibody - Aminoterminal end (ab42078)

All lanes : Anti-DPP9 antibody - Aminoterminal end (ab42078) at 1/5000 dilution

Lane 1 : Molecular weight ladder
Lane 2 : Human DPPII protein
Lane 3 : Human DDPIV protein
Lane 4 : Recombinant DPP8 protein
Lane 5 : DPP9 purified protein from Bovine testis
Lane 6 : Molecular weight ladder

Lysates/proteins at 0.32 µg per lane.

Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 98 kDa
Observed band size : 106 kDa (why is the actual band size different from the predicted?)


Exposure time : 12 minutes

This image is a courtesy of Veronique Dubois

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-DPP9 antibody - Aminoterminal end(ab42078)

IHC image of ab42078 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.