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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-DPP9 antibody - Aminoterminal end
See all DPP9 products (9) ...
Description
Rabbit polyclonal to DPP9 - Aminoterminal end
Specificity
This antibody recognises all three forms of DPP9. This antibody does not recognise other DPP family members.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Synthetic peptide corresponding to the amino terminal of Human DPP9.
(Peptide available as ab44597.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5M Sodium chloride
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 98 kDa.(A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.)
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2.
Tissue specificity
Ubiquitously expressed, with highest levels in liver, heart and muscle, and lowest levels in brain.
Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily.
Cellular localization
Cytoplasm > cytosol.
Target information above from: UniProt accessionQ86TI2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dipeptidyl peptidase IV related protein 2 antibody
- Dipeptidyl peptidase 9 antibody
- Dipeptidyl peptidase IV related protein 2 antibody
see all
Database links
- Entrez Gene: 91039 Human
- Entrez Gene: 224897 Mouse
- Entrez Gene: 301130 Rat
- Omim: 608258 Human
- SwissProt: Q86TI2 Human
- SwissProt: Q8BVG4 Mouse
- Unigene: 515081 Human
- Unigene: 205535 Mouse
see all
Anti-DPP9 antibody - Aminoterminal end images:
Western blot - DPP9 antibody - Aminoterminal end (ab42078)
All lanes : Anti-DPP9 antibody - Aminoterminal end (ab42078) at 1/1000 dilution
Lane 1 : Cell line DU 145 treated with SF
Lane 2 : Cell line DU 145 treated with RA
Lane 3 : Cell line DU 145 treated with IL1-beta
Lane 4 : Cell line DU 145 treated with SF
Lane 5 : Cell line DU 145 treated with IL1-beta
Lane 6 : Cell line DU 145 treated with Progesterone
Lane 7 : Cell line DU 145 treated with Testosterone
Predicted band size : 98 kDa
Observed band size : 98 kDa
Western blot - DPP9 antibody - Aminoterminal end (ab42078)
All lanes : Anti-DPP9 antibody - Aminoterminal end (ab42078) at 1/5000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Human DPPII protein
Lane 3 : Human DDPIV protein
Lane 4 : Recombinant DPP8 protein
Lane 5 : DPP9 purified protein from Bovine testis
Lane 6 : Molecular weight ladder
Lysates/proteins at 0.32 µg per lane.
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 98 kDa
Observed band size : 106 kDa (why is the actual band size different from the predicted?)
Exposure time : 12 minutes
This image is a courtesy of Veronique Dubois
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-DPP9 antibody - Aminoterminal end(ab42078)
IHC image of ab42078 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42078, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-DPP9 antibody - Aminoterminal end (ab42078)
ab42078 has not yet been referenced specifically in any publications.
Publishing research using ab42078? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"